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Suran Rajapakse said on 14 July 2009
"Was it difficult to get to the pipette when you were denuding? Because in certain patients you will find the eggs are but bigger that usual. Unless you have taken the correct approach since you have checked the possible areas. Probably the eggs might be empty Zona which you encounter occasionally. Also one thing more how long have you kept for maturing them before stripping? DId see any evidence of Infections because some times in certain infections such as Beta haemolytic strep the appearance is almost same as empty zona."
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rupture is most probable
alok teotia said on 16 June 2009
"i fully agree with Reeds, the zona fracture is the most probable cause
alok"
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Empty cumulus
Michael L Reed said on 14 June 2009
"From your description, it is hard to tell exactly what you are seeing. Can you identify empty zona pellucidas, or zona fragments after hyaluronidase use and stripping? I've found that ooctyes may fracture during aspiration, despite careful control over the pump pressure, and that oocytes may indeed be intact when they are picked up out of the aspirate, but they have either degenerated by the time stripping takes place, or the stripping destroys the oocytes within the fractured zonas. If zonas are being fractured at retrieval, there could be a pressure problem with the aspiration pump, or the pump pressure being increased to pull difficult follicles, a needle problem, e.g. wrong size needle by mistake, or bad quality control on the needle tip.
I see broken zonas on occasion, but when there are a number of them all of a sudden, then I check the system from start - needles, tubing, aspiration pump - to finish - everything in the laboratory that is used to pipette the oocytes and remove hyaluronidase, as you are doing, no matter."
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