| |
|
IVF > IVF Mail
Answer this question |
View Replies |
Refer it to Friends |
Contact Thaer Abu Hmeidan
|
| Question: |
|
 |
Hi, i have a problem in 2 ivf cases in which i found empty cumulus after the enzymetic denudation, i checked the hyalurinidase with the other patients in the same days that i got the cases and it was ok, i checked the same denudation pipette on oocytes for oher patients and there was nothing wrong with it, plz help?!! thanx |
|
|
|
More Images
|
 Write a reply...
Was it difficult to get to the pipette when you were denuding? Because in certain patients you will find the eggs are but bigger that usual. Unless you have taken the correct approach since you have checked the possible areas. Probably the eggs might be empty Zona which you encounter occasionally. Also one thing more how long have you kept for maturing them before stripping? DId see any evidence of Infections because some times in certain infections such as Beta haemolytic strep the appearance is almost same as empty zona.
Suran Rajapakse - 14 July 2009
rupture is most probable
i fully agree with Reeds, the zona fracture is the most probable cause
alok
alok teotia - 16 June 2009
Empty cumulus
From your description, it is hard to tell exactly what you are seeing. Can you identify empty zona pellucidas, or zona fragments after hyaluronidase use and stripping? I've found that ooctyes may fracture during aspiration, despite careful control over the pump pressure, and that oocytes may indeed be intact when they are picked up out of the aspirate, but they have either degenerated by the time stripping takes place, or the stripping destroys the oocytes within the fractured zonas. If zonas are being fractured at retrieval, there could be a pressure problem with the aspiration pump, or the pump pressure being increased to pull difficult follicles, a needle problem, e.g. wrong size needle by mistake, or bad quality control on the needle tip.
I see broken zonas on occasion, but when there are a number of them all of a sudden, then I check the system from start - needles, tubing, aspiration pump - to finish - everything in the laboratory that is used to pipette the oocytes and remove hyaluronidase, as you are doing, no matter.
Michael L Reed - 14 June 2009
|
|
 |
|
|
