Product Reviews

Embryo Freezing Pack

IVF Journals

RBM Online
ISSN 1472-6491

The Journal of Clinical Embryology
ISSN 1941-1901

Common Questions

IVF > IVF Network

Send me a private message.

Dr. Alex Varghese
Embryologist, Scientific Director

Fertility Clinic & IVF Div., AMRI Medical Center 97A, Southern Avenue CALCUTTA WB 700029 India
Website: http://www.amrihospital.com/clinic/ivf_centre/index.htm
Primary Language: English

About Me

Currently associated with a leading IVF center in India as Scientific Director & Chief Embryologist Founder- www.lifeinvitro.com

Formerly Post doctoral Fellow at Cleveland Clinic, Ohio

My Career History

Working for the last 13 years in the field of clinical IVF and reproductive biology research http://www.clevelandclinic.org/reproductiveresearchcenter/info/collab.html#india

My Research Interests

Cryopreservation of gametes/embryos, effect of environmental toxins on reproduction, role of antioxidants inART

I am interested in collaboration

Areas in which I would like to collaborate include:
Please ask me.

My Interests and hobbies

Travel, movies

My Publications

Varghese AC, Goldberg E, Bhattacharyya AK, Agarwal A (2007) Emerging technologies for the molecular study of infertility, and potential clinical applications, Reproductive Biomedicine Online 15, 4, 451-456.

Varghese AC, Goldberg E and Agarwal A (2007) Current and future perspectives on intracytoplasmic sperm injection (ICSI): A critical commentary Reproductive Biomedicine Online 15, 6, 719-727.

Varghese AC, Sinha B, Bhattacharyya AK, New frontiers in the evaluation of sperm function, in vitro selection and manipulation of male gametes for assisted conception, Indian Journal of ExperimentalBiology, 43, November, 2005, pp.1023-1031

Varghese AC, Stefen du Plessis, Agarwal A (2008) Male Gamete Survival at Stake- causes and solutions, Reproductive Biomedicine Online, 17 (6), 866-880.

Varghese AC, Bragais FM, Mukhopadhyay D, Kundu S, Pal M, Bhattacharyya AK, Agarwal A (2009) Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm quality. Andrologia, 41, 1-9.

Bhattacharya, S.M., Mukhopadhyay, D., Varghese, A.C., Kundu, S., Bhattacharyya, A.K. How does oxidative stress affect human sperm function? Embryo Talk, vol. 1 (2006)

Makker K, Varghese AC, Murandi R, Desai N and Agarwal A (2009) Cell phone usage-Modern man’s nemesis, RBM online, 18, 148-157.

Alex C Varghese, Stephen du Plessis, Tomasso Falcone , Ashok Agarwal (2008) Cryopreservation of ovarian tissues and in vitro follicle culture: challenges for fertility preservation Reproductive Biology & Endocrinology, 6, 47

Alex C Varghese, Peter Nagy and Agarwal A (2009) Current trends, biological foundations and future prospects of human oocyte and embryo cryopreservation Reproductive BioMedicine Online 2009 Vol.19 No.1. 126–140).

Agarwal A, Desai N, Makker K, Varghese A and Murandi R (2008) Effect of cell phone radiation on sperm function, ROS and DNA integrity: an in vitro study. Fertility and Sterility, doi:10.1016/j.fertnstert.2008.08.022 (In press)

Varghese AC, Amir Arsalan and Agarwal A (2009) An update on developments and future prospects of ICSI. Arch Med Sci 2009; 5, 1A: S109–S114

Dyutiman Mukhopadhyay, Parag Nandi, , Alex C. Varghese,, Rohit Gutgutia, , Samir Banerjee, , Asok

K. Bhattacharyya^. (2008) The in-vitro effect of benzo-a-pyrene on human sperm hyperactivation and acrosome reaction. Fertility & Sterility (doi:10.1016/j.fertnstert.2009.02.031 In press)

Dyutiman Mukhopadhyay, Alex C Varghese, Furquan Pathan, Shukla Kundu, Manisha Pal, Parag Nandi, Samir Banerjee and Asok K Bhattacharyya (2008) Effect of age on semen parameters as observed in 2329 men attending an andrology lab in an Indian city. Human Fertility (communicated)

Alex C Varghese, Daniel de Matis, Shabana Sayed, Sakkas Denny, Ashok Agarwal (2008) Novel Strategies and Biological Foundations for selecting human gametes and embryos in Assisted Conception Technologies: A Mini-review. Human Reproduction Update

Kathelene O’Brian, Varghese AC, Agarwal A (2010) Advances in genetics of male infertility. Fertility& Sterility , Jan, 2010

Mukhopadhyay D, Mitra A, Nandi P, Varghese AC, Murmu N, Chowdhury R, Chaudhuri K and Bhattacharyya AK. (2008) Expression of Metallothionein-1 (MT-1) mRNA in the rat testes and liver after Cadmium injection. System Biology in reproductive Medicine (In press).

Mukhopadhyay D, Alex C. Varghese, Manisha Pal, Sudip K. Banerjee, Asok K. Bhattacharyya, Rakesh K. Sharma and Ashok Agarwal, (2009) Semen quality and age-specific changes: A study between two decades on 3729 male partners ofcouples with normal sperm count and attending anandrology laboratory for infertility-related problems in an Indian city. Fertility and Sterility (doi:10.1016/j.fertnstert.2009.01.135 In press)

D. Mukhopadhyay, A. C. Varghese, P. Nandi, S. K. Banerjee & A. K. Bhattacharyya (2009) CASA-based sperm kinematics of environmental risk factor-exposed human semen samples designated as normozoospermic in conventional analysis, Blackwell Verlag GmbH Andrologia 41, 1–5 9( In press)

Varghese AC, Kim Ly, Corbin C, Mendiola J and Agarwal A (2010) Oocyte competence and embryo development: Role of environment and in vivo factors, RBM Online, In press)

Chapters published in Books

Alex C Varghese, Damayanti Chakraborty, A. K. Bhattacharyya. Ovarian tissuecryopreservation, chapter in, Kamini Rao (editor): ART Laboratory manual, Jaypee Medical Publishers

2. Agarwal A, Vaghese AC, Sharma R (2009) Markers of oxidative stress and sperm chromatin integrity.In: Molecular Endocrinology-Methods & protocols, Park-Sarge, Ok-Kyong;Curry, Jr., Thomas (Eds.) , Humana Press, Springer- Verlag

Makker K, Varghese AC, Sharma R and Agarwal A (2008) Interaction between reactive oxygen species and male germ cells. Edts- Glander, Germany (In press)

Alex C Varghese, Furquan Pathan, Jeat Lee (2010). Human Semen Analysis in, Agarwal & Kamini Rao (editor): Andrology Laboratory manual, Jaypee Medical Publishers

Alex C Varghese, Jeat Lee, Furquan Pathan (2010). Physiology of testicular function in, Agarwal A & Kamini Rao (editor): Andrology Laboratory manual, Jaypee Medical Publishers

Kelly Athayde and Alex C Varghese, Agarwal A (2010). Quality control management in Andrology Lab in, Agarwal & Kamini Rao (editor): Andrology Laboratory manual, Jaypee Medical Publishers

Alex C Varghese, Parag Nandi, Reda Mahfous, Kelly Athayde, Ashok Bhattacharyya and Agarwal (2010) Human semen cryopreservation in, Agarwal & Kamini Rao (editor): Andrology Laboratory manual, Jaypee Medical Publishers

Alex C Varghese, Hammadeh Sperm DNA integrity assessment by AOT in Armand Zini, Agarwal A (edt): Human sperm DNA integrity, Humana Press, Springer- Verlag, USA ( in preparation)

Alex C Varghese, Fernandez (2010) Extended embryo culture supplementation in Armand Zini, Agarwal A (edt); ANTIOXIDANTS IN THE MEDICAL AND SURGICAL MANAGEMENT OF MALE INFERTILITY” Humana Press, Springer- Verlag, USA (In preparation)

Alex C Varghese, Nicholas Prados (2010) IVF Work stations, In Peter Nagy, Alex Varghese, A. Agarwal (edt) PRACTICAL MANUAL OF IN VITRO FERTILIZATION: Newer methods and novel devices,SPINGER VERLAG, USA

Books Edited (In preparation & expected release 2011):

PETER NAGY, ALEX C VARGHESE AND ASHOK AGARWAL (Edt) PRACTICAL MANUAL OF IN VITRO FERTILIZATION: Newer methods and novel devices, SPRINGER-VERLAG, USA

ALEX C VARGHESE, PETER BJOBLOM, JAYAPRAKASHAN (Edt) A PRACTICAL GUIDE TO SETTING UP AN IVF LAB AND EMBRYO CULTURE SYSTEMS, JAYPEE MEDICAL PUBLISHERS

My Presentations

Abstracts in International Meetings:

Varghese A C, Bhattacharyya A K, Roy H, Allamaneni S S, Bhattacharyya J, Agarwal A. A comparative study of nuclear DNA integrity and morphology of human spermatozoa processed by three different methods. Fertil Steril 82, Sup 2 (2004) S104.

Pal S, Varghese AC, Agarwal A, Bhattacharyya AK, The effect of temperature and duration of cryopreservation on human sperm characteristics. Fertil Steril 82; Sup 2 (2004)

Varghese AC, Chakraborty D, Bhattacharyya AK. Said TM, Bhattacharya SM, Agarwal A. Enhancement of human sperm motility by inclusion of acetyl-L-carnitine in processing media Fertil Steril 84; Sup.1,(2005)S105.

Bhattacharyya AK, Varghese AC, Das S, Biswas A, Mondal M, Agarwal A. Motion characteristics of frozen-thawed human spermatozoa processed by different methods: a comparative study, Fertil Steril

84; Sup.1,(2005)S177.

Varghese AC, Das S, Bhattacharyya AK, Bhattacharya SM, Mondal M, Agarwal A. Effect of cryoprotective additives- reduced glutathione, acetyl-L-carnitine on sperm membrane lipid peroxidation, DNA integrity and recovery of motile human sperm Fertil Steril 84; Sup.1,(2006)S105.

Bhattacharyya, A.K.; Chakraborty, D.; Varghese, A.C.; Bhattacharyya, S.M.; Kundu, S.; Banerjee, A. Cryopreservation and post-thaw motility, DNA integrity and acrosome status of pre-freeze prepared human semen frozen in different trehalose concentrations. Hum. Reprod. 21; Sup.1,(2006)P-393.

Satyasrini, S.V.; Varghese, A.C.; Chakraborty, D.; Kundu, S.; Vasan, B.; Bhattacharyya, A.K. N-acetyl glucosamine and beta glycerophosphate supplementation in cryopreservation media on post thaw sperm functional characteristics. Hum. Reprod. 21; Sup.1,(2006)P-391.

Vasan, B.; Chakraborty, D.; Satyasrini, V.; Varghese, A.C.; Bhattacharya, S.M.; Bhattacharyya, A.K. Dose dependent effect of cryoprotective agents on post thaw human sperm motility, morphology, lipid peroxidation and DNA integrity. Hum. Reprod. 21; Sup.1,(2006)P-390.

J. Bhattacharya, S. Kundu, A. C. Varghese, S. M. Bhattacharya, A. K. Bhattacharyya, A. Agarwal A comparative study on TEST-Yolk buffer and human sperm preservation medium on post thaw characteristics of human sperm from pre-freeze specimens. Fertil. Steril 2006; 86, (3)

Bhattacharyya, A. C.Varghese, S. M. Bhattacharya, P. Ray, J. Bhattacharya, A. Agarwal A computer assisted sperm motility based study on rapid vs. slow thawing temperatures on human sperm motility parameters. Fertil. Steril 2006; 86, (3) S197

C. Damayandi, A. C. Varghese, D. Mukhopadhaya, S. M. Bhattacharya,K. Bhattacharyya, A. Agarwal Motility characteristics and DNA integrity of spermatozoa processed with different concentrations of reduced glutathione (GSH) Fertil. Steril 2006; 86, (3) S446

Mosammat Begum, David Miller, M A Salam, Ehsam Quadir, Alex C. Varghese, Ashok Agarwal Antibiotics and micronutritional blend to enhance fertility potential in men with abnormal semen quality (Accepted for presentation at 64th Annual Meeting of the American Society for Reproductive Medicine)

Sajal Gupta, Reda Mahfouz, Alex Varghese, Rakesh Sharma, Edmund Sabanegh, Ashok Agarwal Association of sperm DNA fragmentation with seminal oxidative stress in male factor infertility (Submitted to 64th Annual Meeting of the American Society for Reproductive Medicine)

Israel Maldonaldo, Alexandra Bermudez, Alex Varghese, Jose Gaytan, Rakesh Sharma, Ashok Agarwal Oocyte dysmorphism is not a limiting factor for post-thaw survival following vitrification by cryotop method (Accepted for presentation at 64th Annual Meeting of the American Society for Reproductive Medicine)

Asok Bhattacharya, S Suresh, V Vineetha, Bijoy Sebastian, Alex Varghese, Ashok Agarwal Antioxidant effects of Triphala, Brahmi and Triphala- Brahmi combination in blood and seminal plasma of high glucose fed rats (Accepted for presentation at 64th Annual Meeting of the American Society for Reproductive Medicine)

Jaya Bhattacharya, Asok Bhattacharyya, Dyutiman Mukhopadhyaya, Alex Varghese, Ashok Agarwal Declining semen quality in Indian subcontinent-a study spanning two decades (1980-1985 and 2001-2006) (Submitted to 64th Annual Meeting of the American Society for Reproductive Medicine)

Alex. C. Varghese, Gutgutia R, Bhattacharyya AK, Suresh.S, Bhattacharya J, Dasgupta P. ANTIOXIDANT SYSTEM IN SEMINAL PLASMA AND ITS RELATIONSHIP TO SEMEN PARAMETERS IN INFERTILE MEN(Presented at 65th Annual Meeting of the American Society for Reproductive Medicine, Atlanta, Georgia)

Desai, N, Varghese AC et al Antioxidant effects of indian medicinal plants on seminal plasma enzymes of high glucose fed rats ( Presented at the Annual meeting of the American Society of Andrology, Houston, Texas, April, 2010)

Additional photos