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IVF > Image Database > Sperm



Aniline Blue Eosin for Sperm Immaturity


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* For permission to use this image or to obtain a higher quality please contact
Professor Philip J. Chan
© Do not reproduce without permission.

Description:

Aniline blue assay modified by adding eosin counterstain improved detection of immature sperm characterized by lysine-rich histones. Immature sperm stained dark blue while mature sperm stained red. Normal reference was set at > 75% staining red. Fertil Steril 2008:90;p.1999-2002

Submitted by: Contact Professor Philip J. Chan
Loma Linda Univ. School of Medicine, Gyn/Ob Department, Loma Linda, CA 92354, USA

Page Views: 5535

Additional photos

Immature sperm staining dark blue. WBC stained blue as quality control.

Immature bent-neck sperm

Immature coiled-tail sperm

Immature stump-tail sperm

Immature triple-head sperm
 

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