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artifacts in culture dishes.

By: SPG,
24 December 2009

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Question:
	Hi guys. sometimes we see artifacts in our culture dishes. we use BD falcon 7575 to aliquote medium and prepare culture dishes. when you open individually packed 7575 pippettes, lot of particles are produced, which you can see in the image. we suspect we see the same particles in our culture dishes. Anyone having this problem? Pls comment and share your experience on this.

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Artefacts in 7575 pasteur pipettes.
Dear SPG,

It is the problem with packaging. The transfer pets 7575 are packaged as "strips". The individual pipettes are packed on a single sheet of paper, wrapped and sealed by plastic covering. These sheets are then punched to make perforations to facilitate the separation of single pipette. When you tear off one pipette from the strip, the perforated line produces fine paper pieces ( as shown in your picture).

To avoid those tiny paper pieces, I separate the individual pipettes whenever I get the time. Clean them just by jurking or taping or sometimes by wiping with tisuue. Keep these relatively debris free pipettes in my lab for ready use.

If you find the artifacts inside the packing, that could be due to poor handling during transportation.

Hope this helps.

-Sanjay
Sanjay Shukla - 04 January 2010

ARTIFACTS
Dear SPG, the image provided by u is not a regular feature of this product. please check the transport and storage of the same, however the glass pipettes delivers a more consistent volume in each drop and therefore more preferable for dish preparation. artifacts in the culture dish are an indicator for lab hygiene (particulate contaminants- dust), an air purification system with positive pressure inside the lab should help.
ALOK TEOTIA - 28 December 2009