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Cook media correct use

By: ramona,
08 January 2009
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Question:
in your oppinion the correct Cook media to be used after denuding oocytes for ICSI is Cleavage media or Fertilization media? What type of media do you culture after ICSI?
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Shrikant Yatnale


COOk media
Shrikant Yatnale said on 18 January 2009

"After denuding oocytes for ICSI I prefer to keep in Fertilisation media, and ICSI is also done using fertilisation media (minimal exposure during ICSI precedure) At a time only 4 to 5 oocytes are loaded in ICSI dish, so that there is no risk of long exposure.
Post ICSI the cleavage media is used to culture the embryos."

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