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Professional Skills and Attributes Andrology

Routine semen analysis (WHO standards and NATA protocols)Ø

Antisperm antibody testing (MARØ & Immuno bead testing)

Computerised semen analysisØ

Sperm preparation for Various ART techniques (Simple washing, Swim upØ & Gradient techniques)

Sperm preparation after PESA, MESA, TESE and Testicular biopsiesØ

Maturation of sperms from testicular samplesØ

Ionophoric stimulation of sperms with motility problemsØ

Cryopreservation of sperm and Testicular biopsy tissuesØ

Assessment of Testosterone levels by RadioØ

Gamete Handling: Identification of oocytesØImmuno Assay Embryology  from the follicular fluid, oocyte grading (GV, MI & MII stages) In Vitro Fertilisation (standard Insemnination)ØIUI (USG guided) Ø Embryo culture (Extended embryo culture; 90% blastocyt rates, minimalØ Embryo assessment (Gardner’s gradingØfragementation and degradation)  ICSI (trained on different equipments likeØand Sydney IVF system)  Eppendorff, Narishigi and Leitz and also trained in setting up the CompetentØmicromanipulator and making micromanipulation pipettes)  

EmbryoØGIFT and MIFT Ølevel of training and acceptable success rates  cryopreservation (D3 & OocyteØD5; Slow cooling and Vitrification)  LASER assisted hatching of embryosØand Ovarian tissue cryopreservation  Orcein staining andØEmbryo biopsy (blastomere and polar body biopsy) Ø Media preparation for gamete handling, embryoØfixation of embryos  Incubator cleaning, maintainence ofØculture and cryopreservation  Total QualityØvarious lab equipment and sterilisation procedures  management in an IVF lab (QC & Setting up a new IVFØQA procedures)  lab

Documentation of various procedures and Internal Audit in IVF labØ

Trouble shootingØ

Interpretation of PGD results and counselling support for infertile couplesØ

Genetic counselling in high risk casesØ

Tissue culture, maintaining cell lines in culture, serial cell passages (basics of stem cell culturing)Ø

ELISA, Immunohistochemistry and MTS assayØ Research in Reproductive medicine Basic lab skills - tissue culture, Radio immuno assay, animal experiments, ELISA, MTS assay, Immuno histo chemistry, vaginal smear examination, PAP smear. Writing literature reviews and scientific papers

 History

Career History: Experience: Professional Experience -Under took a mini research project at the Centre for Women Health Research, Monash Medical Centre, Australia, 2007. The project was in the lab of Dr. Ursula and it was regarding the role of endothelin-1 in endothelial dysfunction in pre eclamptic cases. The scientific work is still going on. -As a part of of my graduate diploma and masters has undergone extensive vocational training in various aspects of ART and has gained professional level of competency(2007 & 2008) –

Work Experience – Monash IVF Queensland – 7th to 14th July, 2008. As a part of this I got hands on training in various aspects of Human IVF

Education: Educational qualifications Master of Clinical Embryology from Monash University, Australia – 2008Ø Post Graduate Diploma in Reproductive Sciences from Monash University,Ø Australia – 2007 PG Diploma in Hospital Management from AIIMS, Chennai, India – 2006Ø MBBS from Rajiv Gandhi University, Bangalore, India- 2005Ø Training Courses Completed the ‘Liquid Nitrogen Training course’ conducted byØ Occupational Health and Safety Department, Monash University on 1st May 2008 Completed the training in ‘Ergonomics / Manual Handling, DangerousØ Goods & Hazardous Substances & Risk Management’ conducted by Occupational Health and Safety Department, Monash University on 14th March 2008 Completed the ‘Training Course in Laboratory Animal Care and Use 2008’Ø conducted by Animal Welfare Committee, Monash University on 19th February 2008 Professional Registration Registered with Indian Medical Council and Karnataka State Medical Council Registration No. 73414 Year of Registration – 2006

 Personal

Personal details: My strengths Honest, intelligent, creative, perfectionist, strongly emphatic, enjoys a challenge and a self starter Languages known: English, Hindi, Telugu, Kannada, and Marathi (reading and writing)

Interests and Hobbies: Community work I strongly believe that service to humanity is service to god. I have been involved with few of the NGO’s in India in doing community work. I have worked with World Vision and Medi vision. Both the organisations undertake medical camps in the rural areas of India offering medical facilities and treatment. Career Intrests Successful ICSI with primary spermatocytes in Humans. Ovarian tissue transplantation Designer babies Proteomics and metabolomics in ART Therapeutic cloning and Embryonic stem cells

Further Information: Referees: 1. Dr. Peter Temple –Smith Director EPRB Faculty of Medicine, Nursing and Health Sciences Monash Institute of Medical Research (MIMR) Room Bridge, Building Level 3, Monash Medical Centre Contact no - +61 3 9594 7433; mobile - 0400 009 627 Email - Peter.Temple-Smith@med.monash.edu.au 2. Dr. Ursula Manuel Pillai BSc (Hon), PhD Senior Research Fellow CENTRE FOR WOMEN'S HEALTH RESEARCH. MMC, Clayton. Email - ursula.manuelpillai@med.monash.edu.au Contact no - +61 3 95945372 3. Dr. sally Catt Course Co-ordinator Masters in Clinical Embryology Monash Institute of Medical Research Faculty of Medicine, Nursing and Health Sciences Monash Institute of Medical Research (MIMR) Room Bridge, Building Level 3, Monash Medical Centre Email - Sally.Catt@med.monash.edu.au Contact no - +61 3 9594 7434 Dr. Krishna Mantravadi

 Science

Publications: Presentations: Presentations I have done various Literature reviews, Journal Reviews and Poster presentations as a part of the course at Monash University. Poster presentations Poster presentation – topic “Will ICSI replace IVF” – 2007, OctoberØ Ø Poster presentation – topic “Two Embryos- too may?” – 2008, April Both the posters were marked as the best and selected for display in MIMR open day. Journal Reviews and Literature reviews Ø Rate, extent, and modifiers of spermatogenic recovery after hormonal male contraception: an integrated analysis Ø Tissue specific roles of estrogens in human body Ø Monitoring pregnancy in a women with immune disorder Ø PCOS current theories in pathophysiology Ø Benign Hypertrophy of Prostate – current trends Ø Predictive power of semen analysis in male infertility Ø Designing an IVF lab – will GMP standards apply??? Ø ICSI is passing on infertile genes to the future- will it be a problem by 2030 Ø Role of ART in conservation of endangered species Ø Male contraception recent advances Ø Role of tTAF’s in spermatogenesis Ø Cortical granule exocytosis after vitrification in oocytes Ø Long term consequences of ICSI Ø Role of Activins and Endothelins in Pre Eclampsia