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IVF > Image Database > Cell Staining



Dual Stain Analysis of Sperm Apoptosis and Necrosis


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Description:
Sperm stained with both bisbenzimide (Hoechst 33342)and propidium iodide fluoresced red when they are necrotic (N; dead), all blue when apoptotic (A; dying) or partial blue when alive. Am J Obstet Gynecol 2003:188;1156-1157.
Submitted by: Contact Professor Philip J. Chan
Loma Linda Univ. Center for Fertility & IVF, 11370 Anderson St., Loma Linda, CA 92354, USA.

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Sperm stained with both bisbenzimide (Hoechst 33342)and propidium iodide fluoresced red when they are necrotic (N; dead), all blue when apoptotic (A; dying) or partial blue when alive. Am J Obstet Gynecol 2003:188;1156-1157.
 

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