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perform ICSI
Mohammed said on 07 June 2010
"If you were quick in performing the ICSI, you can just do it using non-hepes media. otherwise, it is preferreble to do it in hepes buffer media.
i personally do it in hepes buffer media"
Reply
HEPES is better
Rania .J said on 31 May 2010
"Dear Dhana ;
It's better to use HEPES because you are preforming ICSI with no CO2 conditions. Fertilisation media needs CO2"
Reply
icsi media
Orestes said on 26 May 2010
"Use mops or htf with hepes.
They give similar results,"
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Embryologist
R. KRISHNAMOORTHY said on 26 May 2010
"I prefer you to use HEPES medium for ICSI, Because there is no pH is needed for HEPES buffer and also its a best buffer too. When u are using fertilization medium, u should complete ICSI within a short period of time. Using HEPES medium is ideal one."
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