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Immobilization of sperm and injecting time

By: KRISHNAMOORTHY,
28 January 2012
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Question:

Dear all,

I have some doubts regarding immobilization of sperm before ICSI, 
*After immobilization of sperm, within how many mins/sec to inject into the cytolplasm?
*May i select & immobilize sperm and shift to another parallel PVP drop as like IMSI procedure then do the ICSI?
*If I'm delaying the ICSI procedure after immobilization, this it will make any difference in fertilization?
I've this idea in my mind to minimize the exposure of oocytes... Normally i took 10-15 mins for 10 oocytes to inject the sperm..

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