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Turgay Barut said on 16 March 2010

"Hi, you can solve your problem with swim-up techniques or gradient technique.; firstly liquefaction time is very important that's why you must wait enough. When the sample is ready study sperm number and wash with medium appropriate centrifuge speed. Later you must be careful while taking the supernatant. And nicely add new wash solution. You must take your sample to the incubator and 45 degree angle and wait min. 45 minute. However be carefull your tube's cover NOT fully closed. And take 50 microliter nicely and look on the chamber. Later you can collect very very complaisantly swimming sperm cells to new tube (0.8 cc). And nicely inject to patient.
If it does not work you can apply first gradient technique plus swim-up techniques.
My respects."

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