|24 January 2017 by Zuzana Holubcova|
Dear all, could you please share your experince with automatic witness system/IVF matcher you use? Are you happy with bar codes (IMT) or do you prefer RFID technology (RI witness system)? Thanks for any thoughts and suggestions.
|11 January 2017 by J .ford|
IVF lab equipment used great prices pick up in Tri State NYC area .
Ivf office closed in queens /left in storage
heracell 150 total 6
small incubators total 6
cryo pressed sealer
hystersocopy machine storkz
midmark 75 exam table
ohaus analytical plus
Isotemo 1025 and 2025 fisher scientific
|02 December 2016 by Saiprasad|
We recently treated a patient who had a history of Fragile Eggs. In all her cycles most of the Eggs Degenerate after ICSI and has a very low fertilisation rate.
So her Cycle in our Clinic wasn't different. we got 10 Eggs for her.
We incubated the Eggs post ovum pick up for 4 hours, as sometimes the fragility of the eggs is due to cytoplasmic immaturity and long Incubation of OCCs helps in most of such cases.
While performing ICSI, these are the things that i observed.
1) 8 of the eggs were Mii and 2 eggs were mi
2) Almost all eggs had soft membrane and the Injection Needle was going in very easily. Hence i was very careful while aspirating the cytoplasm. Made sure that i aspirated very little cytoplasm, as aspirating the cytoplasm aggressively leads to killing the egg.
3) There was no immediate degeneration. But when i checked the Eggs after ICSI, 6 of them had degenerated.
4) Only 3 Eggs fertilised, when i checked the Next day.
5) Day 2 Gradings were 2x 3-cell grade B, 1x 3-Cell grade C
What is the Right approach, while handling such Eggs
Since the memrane was Soft, Should we not aspirate the cytoplasm at all ?
But by doing that we may end up with lot of failed fertilisation
Please share your Experience.
|31 October 2016 by Manar Hozayen|
would anyone recommend for me a good blood gas analyser for pH measurments, or any other more accurate device???
|06 May 2016 by tagreed shehab|
Please can you tell me if can I freeze embryos after PGD?
|27 April 2016 by Kone|
I am using Global Total (Life global group) media for embryo culture (until day 2/3) and for embryo transfer.
I hear that embryoglue (Vitrolife) improve implantation and life birth baby rates. But mixing two media is not recommended in IVF procedures.
Can I use Global Total for culture and embryoglue for transfer safely? that will not affect my results?
|11 April 2016 by zz|
Could you recommend me round bottom tubes (17x100) with certificate EC ??
I will be very grateful for your help.
|12 January 2016 by J. Hackworth|
Has anyone else had issues with Origio equipment that was sold as new but is obviously used and/or has problems?
|09 January 2016 by Eman Saber|
I am currently doing Master degree and doing a survey “Sperm DNA fragmentation: where do we stand”. I would be really grateful if you can help me with answering this survey, I would like to increase the feedback about this survey to increase its validity.
|20 November 2015 by Koray YILDIZ|
I have seen rarely poor oocyte which has a spesific anomally that has large halo at the periphery. As an embryologist, I made 5 day culture and there is no blastocyst occurence and resulted canceling the ICSI cycle. I am wondering the prognose of this kind of oocytes.
Here is some details;
29 years of woman,third ICSI trial, first two trial were at different good quality laboratories.
Woman Day 3 FSH: 9
E2 : 55
Man: Normal Sperm parameters
Antagonist protocol starting 150 IU rFSH up to day8. At day 4 gnrh antagonist started for 6 days up to day 9.At day 9, 75 IU rFSH and Decapeptyle injected. 35 hours later we collected 8 oocytes( 7 MII, 1 GV). 3 hours after oocyte pickup ICSI made and 5 of them fertilised and cultered 5 days but no blastocyst occured and cycle canceled.
Here is the oocyte and day 5 pictures.
I 'll be very appreciated for your helps,
Dogufertil IVF Center
Malatya / Turkiye