|02 December 2016 by Saiprasad|
We recently treated a patient who had a history of Fragile Eggs. In all her cycles most of the Eggs Degenerate after ICSI and has a very low fertilisation rate.
So her Cycle in our Clinic wasn't different. we got 10 Eggs for her.
We incubated the Eggs post ovum pick up for 4 hours, as sometimes the fragility of the eggs is due to cytoplasmic immaturity and long Incubation of OCCs helps in most of such cases.
While performing ICSI, these are the things that i observed.
1) 8 of the eggs were Mii and 2 eggs were mi
2) Almost all eggs had soft membrane and the Injection Needle was going in very easily. Hence i was very careful while aspirating the cytoplasm. Made sure that i aspirated very little cytoplasm, as aspirating the cytoplasm aggressively leads to killing the egg.
3) There was no immediate degeneration. But when i checked the Eggs after ICSI, 6 of them had degenerated.
4) Only 3 Eggs fertilised, when i checked the Next day.
5) Day 2 Gradings were 2x 3-cell grade B, 1x 3-Cell grade C
What is the Right approach, while handling such Eggs
Since the memrane was Soft, Should we not aspirate the cytoplasm at all ?
But by doing that we may end up with lot of failed fertilisation
Please share your Experience.
|31 October 2016 by Manar Hozayen|
would anyone recommend for me a good blood gas analyser for pH measurments, or any other more accurate device???
|01 September 2016 by Max castor|
Surplus from doctors office in queens , ny
Ivf lab machines and supplies
cryo press sealer
heracell incubator 150
isotemp 1025- fisher scientific
isotemp 2025-fisher scientific
ohaus analytical plus
petrie dishes falcon 35x 10 35-1008 model
petri dish 50x9
falcon 35-3002 petri
|06 May 2016 by tagreed shehab|
Please can you tell me if can I freeze embryos after PGD?
|27 April 2016 by Kone|
I am using Global Total (Life global group) media for embryo culture (until day 2/3) and for embryo transfer.
I hear that embryoglue (Vitrolife) improve implantation and life birth baby rates. But mixing two media is not recommended in IVF procedures.
Can I use Global Total for culture and embryoglue for transfer safely? that will not affect my results?
|11 April 2016 by zz|
Could you recommend me round bottom tubes (17x100) with certificate EC ??
I will be very grateful for your help.
|12 January 2016 by J. Hackworth|
Has anyone else had issues with Origio equipment that was sold as new but is obviously used and/or has problems?
|09 January 2016 by Eman Saber|
I am currently doing Master degree and doing a survey “Sperm DNA fragmentation: where do we stand”. I would be really grateful if you can help me with answering this survey, I would like to increase the feedback about this survey to increase its validity.
|20 November 2015 by Koray YILDIZ|
I have seen rarely poor oocyte which has a spesific anomally that has large halo at the periphery. As an embryologist, I made 5 day culture and there is no blastocyst occurence and resulted canceling the ICSI cycle. I am wondering the prognose of this kind of oocytes.
Here is some details;
29 years of woman,third ICSI trial, first two trial were at different good quality laboratories.
Woman Day 3 FSH: 9
E2 : 55
Man: Normal Sperm parameters
Antagonist protocol starting 150 IU rFSH up to day8. At day 4 gnrh antagonist started for 6 days up to day 9.At day 9, 75 IU rFSH and Decapeptyle injected. 35 hours later we collected 8 oocytes( 7 MII, 1 GV). 3 hours after oocyte pickup ICSI made and 5 of them fertilised and cultered 5 days but no blastocyst occured and cycle canceled.
Here is the oocyte and day 5 pictures.
I 'll be very appreciated for your helps,
Dogufertil IVF Center
Malatya / Turkiye
|17 November 2015 by Saiprasad Gunde|
We Recently had a very poor blastocyst conversion rate for one of our patient, in her 2 cycles.
1st cylce : Only 2 became blastocysts out of 12 Embryos (10 top quality Embryos on day 3)
2nd cycle : Only 2 became blastocysts out of 20 Embryos (14 top quality Embryos on day 3)
Interestingly Blastocysts form both cycles looked very identical.
Have attached pictures.
The fertilisation Rate was excellent.
The embryos looke good till day 3. But then got arrested.
Any body had such experience?
Sperm has a role?
Kindly share your experient.