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1. FRAGILE EGGS. PLEASE SHARE YOUR EXPERIENCE.

02 December 2016 by Saiprasad

HI,

We recently treated a patient who had a history of Fragile Eggs. In all her cycles most of the Eggs Degenerate after ICSI and has a very low fertilisation rate.

So her Cycle in our Clinic wasn't different. we got 10 Eggs for her.

We incubated the Eggs post ovum pick up for 4 hours, as sometimes the fragility of the eggs is due to cytoplasmic immaturity and long Incubation of OCCs helps in most of such cases.

While performing ICSI, these are the things that i observed.

1) 8 of the eggs were Mii and 2 eggs were mi

2) Almost all eggs had soft membrane and the Injection Needle was going in very   easily. Hence i was very careful while aspirating the cytoplasm. Made sure that i aspirated very little cytoplasm, as aspirating the cytoplasm aggressively leads to killing the egg.

3) There was no immediate degeneration. But when i checked the Eggs after ICSI, 6 of them had degenerated.

4) Only 3 Eggs fertilised, when i checked the Next day.

5) Day 2 Gradings were  2x 3-cell grade B, 1x 3-Cell grade C

What is the Right approach, while handling such Eggs

Since the memrane was Soft, Should we not aspirate the cytoplasm at all ?

But by doing that we may end up with lot of failed fertilisation

Please share your Experience.

Thank you,

 

 

 

 


        Replies (0)

2. pH measurment

31 October 2016 by Manar Hozayen

would anyone recommend for me a good blood gas analyser for pH measurments, or any other more accurate device??? 


        Replies (0)

3. used ivf machines and new supplies for sale

01 September 2016 by Max castor

Surplus from doctors office in queens , ny

Ivf lab machines and supplies 

cryo press sealer

heracell incubator 150

isotemp 1025- fisher scientific 

isotemp 2025-fisher scientific

co incubator 

ohaus analytical plus

 

petrie dishes falcon 35x 10 35-1008 model

petri dish 50x9 

falcon 35-3002 petri 

and supplies 

 

 


        Replies (6)

4. PGD

06 May 2016 by tagreed shehab

Please can you tell me if can I freeze embryos after PGD?


        Replies (3)

5. Global total (Life global group) and Embryoglue (Vitrolife)

27 April 2016 by Kone

Dear, 

I am using Global Total (Life global group) media for embryo culture (until day 2/3) and for embryo transfer.

I hear that embryoglue (Vitrolife) improve implantation and life birth baby rates. But mixing two media is not recommended in IVF procedures.

Can I use Global Total for culture and embryoglue for transfer safely? that will not affect my results?

Regards 


        Replies (1)

6. round bottom tubes with EC

11 April 2016 by zz

Dear All

Could you recommend me round bottom tubes (17x100) with certificate EC ??

I will be very grateful for your help.

Best regards,

 


        Replies (0)

7. Origio

12 January 2016 by J. Hackworth

Has anyone else had issues with Origio equipment that was sold as new but is obviously used and/or has problems?


        Replies (1)

8. Sperm DNA fragmentation testing, where do we stand?

09 January 2016 by Eman Saber

I am currently doing Master degree and doing a survey “Sperm DNA fragmentation: where do we stand”. I would be really grateful if you can help me with answering this survey, I would like to increase the feedback about this survey to increase its validity.
No doubt that upon completion of this survey the results and data analysis will be mailed back.

The survey can be accessed through: https://www.surveymonkey.com/r/spermdnafrag

Thank you in advance

 


        Replies (0)

9. Case report-Poor Oocyte Quality

20 November 2015 by Koray YILDIZ

Dear Colleagues,

      I have seen rarely poor oocyte which has a spesific anomally that has large halo at the periphery. As an embryologist, I made 5 day culture and there is no blastocyst occurence and resulted canceling the ICSI cycle. I am wondering the prognose of this kind of oocytes.

Here is some details;

29 years of woman,third ICSI trial, first two trial were at  different good quality laboratories.

Woman Day 3 FSH: 9

         LH: 4,5

         E2 : 55

         Prolactine: 10,2

         TSH: 2,8

         HSG: Normal

       Man: Normal Sperm parameters

Antagonist protocol starting 150 IU rFSH up to day8. At day 4 gnrh antagonist started for 6 days up to day 9.At day 9, 75 IU rFSH and Decapeptyle injected. 35 hours later we collected 8 oocytes( 7 MII, 1 GV). 3 hours after oocyte pickup ICSI made and 5 of them fertilised and cultered 5 days but no blastocyst occured and cycle canceled.

Here is the oocyte and day 5 pictures.

I 'll be very appreciated for your helps,

Thank you.

Koray YILDIZ

Dogufertil IVF Center

Malatya / Turkiye 

 

Case report-Poor Oocyte Quality
        Replies (0)

10. POOR BLASTOCYST CONVERSION RATE

17 November 2015 by Saiprasad Gunde

HI GUYS,

We Recently had a very poor blastocyst conversion rate for one of our patient, in her 2 cycles.

1st cylce : Only 2 became blastocysts out of 12 Embryos (10 top quality Embryos on day 3)

2nd cycle : Only 2 became blastocysts out of 20 Embryos (14 top quality Embryos on day 3)

Interestingly Blastocysts form both cycles  looked very identical.

Have attached pictures.

The fertilisation Rate was excellent.

The embryos looke good till day 3. But then got arrested.

Any body had such experience?

Sperm has a role?

Kindly share your experient.

Thank you,

 

POOR BLASTOCYST CONVERSION RATE
        Replies (1)


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