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IVFMAIL is a questions and answers forum for scientists & physicians. Ask your questions here.

1. PGD

06 May 2016 by tagreed shehab
Please can you tell me if can I freeze embryos after PGD?
        Replies (2)

2. Global total (Life global group) and Embryoglue (Vitrolife)

27 April 2016 by Kone
Dear, I am using Global Total (Life global group) media for embryo culture (until day 2/3) and for embryo transfer. I hear that embryoglue (Vitrolife) improve implantation and life birth baby rates. But mixing two media is not recommended in IVF procedures. Can I use Global Total for culture and embryoglue for transfer safely? that will not affect my results? Regards
        Replies (0)

3. round bottom tubes with EC

11 April 2016 by zz
Dear All Could you recommend me round bottom tubes (17x100) with certificate EC ?? I will be very grateful for your help. Best regards,
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4. Origio

12 January 2016 by J. Hackworth
Has anyone else had issues with Origio equipment that was sold as new but is obviously used and/or has problems?
        Replies (1)

5. Sperm DNA fragmentation testing, where do we stand?

09 January 2016 by Eman Saber
I am currently doing Master degree and doing a survey “Sperm DNA fragmentation: where do we stand”. I would be really grateful if you can help me with answering this survey, I would like to increase the feedback about this survey to increase its validity. No doubt that upon completion of this survey the results and data analysis will be mailed back. The survey can be accessed through: https://www.surveymonkey.com/r/spermdnafrag Thank you in advance
        Replies (0)

6. Case report-Poor Oocyte Quality

20 November 2015 by Koray YILDIZ
Dear Colleagues, I have seen rarely poor oocyte which has a spesific anomally that has large halo at the periphery. As an embryologist, I made 5 day culture and there is no blastocyst occurence and resulted canceling the ICSI cycle. I am wondering the prognose of this kind of oocytes. Here is some details; 29 years of woman,third ICSI trial, first two trial were at different good quality laboratories. Woman Day 3 FSH: 9 LH: 4,5 E2 : 55 Prolactine: 10,2 TSH: 2,8 HSG: Normal Man: Normal Sperm parameters Antagonist protocol starting 150 IU rFSH up to day8. At day 4 gnrh antagonist started for 6 days up to day 9.At day 9, 75 IU rFSH and Decapeptyle injected. 35 hours later we collected 8 oocytes( 7 MII, 1 GV). 3 hours after oocyte pickup I.. Case report-Poor Oocyte Quality
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18 November 2015 by Saiprasad Gunde
HI GUYS, We Recently had a very poor blastocyst conversion rate for one of our patient, in her 2 cycles. 1st cylce : Only 2 became blastocysts out of 12 Embryos (10 top quality Embryos on day 3) 2nd cycle : Only 2 became blastocysts out of 20 Embryos (14 top quality Embryos on day 3) Interestingly Blastocysts form both cycles looked very identical. Have attached pictures. The fertilisation Rate was excellent. The embryos looke good till day 3. But then got arrested. Any body had such experience? Sperm has a role? Kindly share your experient. Thank you, POOR BLASTOCYST CONVERSION RATE
        Replies (1)

8. SAGE 1-Step Medium

23 September 2015 by Davor Štimac
Hi! Does anyone have experience with SAGE 1-Step Medium? I'm interested about what concentration of CO2 must be in incubator,beacuse in manual is not strictly defined, just say equilibrate for a minimum of 2h in 5-6% CO2?
        Replies (1)

9. lab air

11 September 2015 by ellen
Dear colleagues, We are going to upgrade our lab in China, and would like to renovate our air system. The space is about 2000 square feet (including lab and ORs). We would much appreciate your recommendation in choosing a lab air system design company or an existing system. Any company interested can also contact me at shellen01@yahoo.com Thank you in advance!
        Replies (0)

10. Issue in embryo culture

07 September 2015 by Muhammad Ameen Jmaal
Dear Collegues, We have ab issue regarding mouse embryo culture. Actuially we go for laboratory renovation with some paints after that we started work but after 6 month continous struggle we still can't sort out the pollutants. We purchased the growth media istead of manual formulation but can't obtain any results. Need suggestion of all collegues
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