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IVFMAIL is a questions and answers forum for reproductive scientists & embryologists. Ask your questions here.

621. Problems with mineral oil

06 May 2004 by Michael L. Reed
A few questions for the group, and for the companies that supply us with our finished products (I know many of the comapanies surf these discussion groups):

1) There was a message posted on EmbryoMail a while back about problems with one lot number of mineral oil from Vitrolife - and now questions regarding mineral oil from Sage. Are the companies that supply us with finished products purchasing stocks from the same source, so are these two 'problems' related? What about the containers used for the final product? Is glass or plastic better, or does it not matter? Which type of container is more vuneralbe to shipping, or storage conditions?

2) While a few of the mineral oil products are filter sterilized, they may not be 'washed' in any fashion - I believe that VitroLife sells washed, sterile oil. An old question, but how many of us process the mineral oil in our labs - by some washing technique - before use? I still wash all the mineral oil, more out of habit - but I can't claim that my washing the oil has prevented getting a random 'bad' bottle of mineral oil, as I don't test the oil pre- and post-washing.

3) How many of us test all products, even though these products come to us already screened by bioassays?

4) How exactly do the companies test the products that they sell - which bioassay is better? Are the source materials tested before and after processing, and again randomly after packaging? And what controls are used to evaluate possible shipping and storage conditions?

We take a lot for granted - most of us now purchase our products rather than compound them ourselves in the lab, so we have to be able to trust our sources and let go of some of the responsiblity. And while this is a good forum for discussion these type of problems, we still need to be careful in how we present these problems - are they random, or actual?

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622. Allele specific gene inactivation

06 June 2004 by MUKESH GUPTA
Does anyone has any information on artificial induction of genome-wide allele specific gene inactivation in cells/embryos? I would be glad, if you can share that with me.

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Dear colleagues

I need some information / guidance regarding purchase of instrument for assisted hatching (PGD) programme. Thanks in advance

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624. Serodiscordant couples

29 April 2004 by Dr Abayomi Ajayi
I am having an increasing number of serodiscordant couples,can anyone with experience in sperm preparation for such couples help me with the details?
Dr Ajayi
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625. Globozoospermia, ICSI, and Egg Activation

29 April 2004 by dr.m.jehad
Please tell me what is the answers for I have only elongatid spermatid cells and need to ICSI by spermatid cell ( elongatid or stage c )
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626. Embryo Biopsy

29 April 2004 by Harold Rodriguez
I'm looking for data on blastulation rates as well as implantation and viable pregnancy rates for human embryos undergoing blastomere biopsy when 1 vs. 2 blastomeres are removed at the 5 to 8-cell stages on day 3.
Any info is greatly appreciated.
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627. Gestational sacs with no heartbeat

29 April 2004 by Linda Hoover
Regarding the use of the SAGE oil for overlay in IVF culture and the incidence of a higher number of gestational sacs occurring without heartbeat; can anyone relay the reason the heartbeats would fail to develop in such circumstances? Has anyone had this happen for other reasons in IVF?
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628. IVF culture oil

18 April 2004 by Eva U
There has been recently a concern on embryomail about a bad lot of oil from one company(not named). My question: how about Sage oil? We have seen large number of chemical pregnancies(80%), sac no heartbeat last month- has anyone else seen such results?(We use quinn's fert/clvg, SSS(Irvine), and Sage oil). Thanks
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629. Mixed gas incubators

13 April 2004 by MAYS ALADHAM
Most IVF labs use CO2 incubators for culturing embryos. I am wondering about incubators supplied with mixed gas together with CO2 ,have the latter any privilage over the first ,and which is recommended for embryo culturing.

Thanks for considering my request , i hope if anybody has an answer to give us a hand
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630. Several questions about mouse IVF

05 April 2004 by Lei Pan
Does anyone know how to prepare sperms for mouse IVF?I just release them from epidilymis to IVF medium to let them capacitate.Would centrifugation be a better way to get the best sperms?
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