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621. Abnormal oocytes

18 May 2004 by sathya
please clarify:
1. significance of centrally pitted oocytes.
2.significances of SER+ oocytes.
3.why such poor pregnancy rates when these conditions are present?
4. how do we counsel couples with these oocytes regarding the next cycle if the first attempt fails?
        Replies (1)

622. Sticky eggs

18 May 2004 by Nicola Monks
We recently made the following observations in a case with IVF and ICSI due to mild male factors. The woman is aged 39 with otherwise unexplained infertility and a good response to stimulation - 13 eggs collected. Her partner has a successful vasectomy reversal with mild reduction in sperm count and morphology. Therefore we used 6 eggs for ICSI and 7 for IVF. When denuding for ICSI, the eggs all became stuck together on exposure to hyaluronidase (Medicult Hyadase) and were difficult to dissect free. Next day, the IVF eggs were also stuck together and difficult to dissect: they behaved in the same way as the ICSI eggs from the day before. The results were fine - good fertilisation with both IVF and ICSI, sadly no pregnancy ensued following transfer of 2 "good" IVF embryos. Has anyone else seen a similar sticky effect? Is it likely to be repeated in subsequent cycles? Does it have any connection with a clinical feature such as autoantibodies in the woman? Clinically, she suffers from hypothyroidism and has to take thyroid hormone - I believe this is an autoimmune condition in her case: is there any connection? Any comments gratefully received. Many thanks Nicola Monks
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623. Sperm HBA test

06 May 2004 by grace gu
I am interested in Sperm HBA, the new sperm test kit. However, I have not so much information about it yet.

1. How does it work? In other words, could this test indicate the ability of sperm binding with oocyte directly and properly?
2.How many IVF centers in USA have used this test as a routine test?
3.Could someone send to me a copy of the articles about this test?
        Replies (1)

624. Problems with mineral oil

06 May 2004 by Michael L. Reed
A few questions for the group, and for the companies that supply us with our finished products (I know many of the comapanies surf these discussion groups):

1) There was a message posted on EmbryoMail a while back about problems with one lot number of mineral oil from Vitrolife - and now questions regarding mineral oil from Sage. Are the companies that supply us with finished products purchasing stocks from the same source, so are these two 'problems' related? What about the containers used for the final product? Is glass or plastic better, or does it not matter? Which type of container is more vuneralbe to shipping, or storage conditions?

2) While a few of the mineral oil products are filter sterilized, they may not be 'washed' in any fashion - I believe that VitroLife sells washed, sterile oil. An old question, but how many of us process the mineral oil in our labs - by some washing technique - before use? I still wash all the mineral oil, more out of habit - but I can't claim that my washing the oil has prevented getting a random 'bad' bottle of mineral oil, as I don't test the oil pre- and post-washing.

3) How many of us test all products, even though these products come to us already screened by bioassays?

4) How exactly do the companies test the products that they sell - which bioassay is better? Are the source materials tested before and after processing, and again randomly after packaging? And what controls are used to evaluate possible shipping and storage conditions?

We take a lot for granted - most of us now purchase our products rather than compound them ourselves in the lab, so we have to be able to trust our sources and let go of some of the responsiblity. And while this is a good forum for discussion these type of problems, we still need to be careful in how we present these problems - are they random, or actual?

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625. Allele specific gene inactivation

06 June 2004 by MUKESH GUPTA
Does anyone has any information on artificial induction of genome-wide allele specific gene inactivation in cells/embryos? I would be glad, if you can share that with me.

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Dear colleagues

I need some information / guidance regarding purchase of instrument for assisted hatching (PGD) programme. Thanks in advance

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627. Serodiscordant couples

29 April 2004 by Dr Abayomi Ajayi
I am having an increasing number of serodiscordant couples,can anyone with experience in sperm preparation for such couples help me with the details?
Dr Ajayi
        Replies (1)

628. Globozoospermia, ICSI, and Egg Activation

29 April 2004 by dr.m.jehad
Please tell me what is the answers for I have only elongatid spermatid cells and need to ICSI by spermatid cell ( elongatid or stage c )
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629. Embryo Biopsy

29 April 2004 by Harold Rodriguez
I'm looking for data on blastulation rates as well as implantation and viable pregnancy rates for human embryos undergoing blastomere biopsy when 1 vs. 2 blastomeres are removed at the 5 to 8-cell stages on day 3.
Any info is greatly appreciated.
        Replies (0)

630. Gestational sacs with no heartbeat

29 April 2004 by Linda Hoover
Regarding the use of the SAGE oil for overlay in IVF culture and the incidence of a higher number of gestational sacs occurring without heartbeat; can anyone relay the reason the heartbeats would fail to develop in such circumstances? Has anyone had this happen for other reasons in IVF?
        Replies (3)

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