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621. Clinical oocyte activation

02 June 2004 by Rafel Busc? Quadrada
We have a fertilization failure after ICSI in a case of globozoospermia and we want to repeat the treatment with oocyte activation using calcium ionophore.
Please, someone can tell me wich is the protocol to induce artificial oocyte activation in cases of ICSI with globozoospermic sperm injection?
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622. Degeneration of cleaving embryos

02 June 2004 by Artem Beskov
Dear colleagues, Recently we came across an unusual case of complete cleavage failure. Three embryos generated by ICSI of 4 oocytes (one failed to fertilize) were at 2-cell stage and looked absolutely normal (except uneven size of blastomeres in one of them) when checked on day 2, but on day 3 all the three still had 2 blastomeres that looked dark, granulated and a little shrinked. The oocytes at injection showed SER aggregation. The male partner has oligoasthenozoospermia with marked agglutination, MAR test in the "grey zone", both partners have elevated antisperm antibodies levels in blood. After previous ICSI attempt in another clinic they were told that "some problems at fertilization occurred".

All the other patients' embryos present at that same time in the equal conditions showed normal development and fertilization, so suboptimal culture environment is very unlikely. It seems that fertilization failure or a common-like development arrest would be easier to explain. Has anyone observed the same picture of total degeneration of cleaving embryos in one particular patient and managed to establish any links to clinical or in vitro culture features (occupational hazard, autoimmunological state, SER aggregation, type of media, etc.)?

Thank you for attention, I will gladly provide details should anyone get interested in the case.
        Replies (1)

623. PCOS

02 June 2004 by M. AREF
I have a pcos patient, 24 years (day 3 LH=32 and FSH=4 RATIO 8), on flare protocol, who started injection decapeptyl 0.1 mg sc plus 150 IU HMG IM from cycle day 5. ON stimulation day 6 E2 was high (6300 pgm) and it was decided to stop HMG and continue with decapeptyl daily and repeat E2 assay daily (COASTING). on coasting day 7 E2 is still very high ( more than 30.000 pgm) and vaginal US showing 15-20 follicles in each ovary, leading 26 mm in diameter. I decided to cancel the cycle. ANY COMMENTS PLEASE.
        Replies (3)

624. in vitro maturation of germ cells

02 February 2004 by Sujatha Sivaram
I would like to have some information on the feasibility of invitro maturation of germ cells to spermatid/ elongated spermatids. Has this procedure combined with ICSI resulted in live births? Thank you.
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625. Sterilization and care of IVF CO2 air Incubators

21 May 2004 by Giovanni Vizziello
I would like to now the ideal procedure of sterilization and care of IVF CO2 air Incubators (Sanyo In Cu Sa Fe).
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626. Injection (ICSI) of oocyte without zona

18 May 2004 by Shaheen Panjwani
Does anyone have experience of injecting (ICSI) an oocyte without zona (only cytoplasm). What are its survival and growth chances and is there any literature or published paper available?
        Replies (5)

627. Abnormal oocytes

18 May 2004 by sathya
please clarify:
1. significance of centrally pitted oocytes.
2.significances of SER+ oocytes.
3.why such poor pregnancy rates when these conditions are present?
4. how do we counsel couples with these oocytes regarding the next cycle if the first attempt fails?
        Replies (1)

628. Sticky eggs

18 May 2004 by Nicola Monks
We recently made the following observations in a case with IVF and ICSI due to mild male factors. The woman is aged 39 with otherwise unexplained infertility and a good response to stimulation - 13 eggs collected. Her partner has a successful vasectomy reversal with mild reduction in sperm count and morphology. Therefore we used 6 eggs for ICSI and 7 for IVF. When denuding for ICSI, the eggs all became stuck together on exposure to hyaluronidase (Medicult Hyadase) and were difficult to dissect free. Next day, the IVF eggs were also stuck together and difficult to dissect: they behaved in the same way as the ICSI eggs from the day before. The results were fine - good fertilisation with both IVF and ICSI, sadly no pregnancy ensued following transfer of 2 "good" IVF embryos. Has anyone else seen a similar sticky effect? Is it likely to be repeated in subsequent cycles? Does it have any connection with a clinical feature such as autoantibodies in the woman? Clinically, she suffers from hypothyroidism and has to take thyroid hormone - I believe this is an autoimmune condition in her case: is there any connection? Any comments gratefully received. Many thanks Nicola Monks
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629. Sperm HBA test

06 May 2004 by grace gu
I am interested in Sperm HBA, the new sperm test kit. However, I have not so much information about it yet.

1. How does it work? In other words, could this test indicate the ability of sperm binding with oocyte directly and properly?
2.How many IVF centers in USA have used this test as a routine test?
3.Could someone send to me a copy of the articles about this test?
Thanks.
Grace
        Replies (1)

630. Problems with mineral oil

06 May 2004 by Michael L. Reed
A few questions for the group, and for the companies that supply us with our finished products (I know many of the comapanies surf these discussion groups):

1) There was a message posted on EmbryoMail a while back about problems with one lot number of mineral oil from Vitrolife - and now questions regarding mineral oil from Sage. Are the companies that supply us with finished products purchasing stocks from the same source, so are these two 'problems' related? What about the containers used for the final product? Is glass or plastic better, or does it not matter? Which type of container is more vuneralbe to shipping, or storage conditions?

2) While a few of the mineral oil products are filter sterilized, they may not be 'washed' in any fashion - I believe that VitroLife sells washed, sterile oil. An old question, but how many of us process the mineral oil in our labs - by some washing technique - before use? I still wash all the mineral oil, more out of habit - but I can't claim that my washing the oil has prevented getting a random 'bad' bottle of mineral oil, as I don't test the oil pre- and post-washing.

3) How many of us test all products, even though these products come to us already screened by bioassays?

4) How exactly do the companies test the products that they sell - which bioassay is better? Are the source materials tested before and after processing, and again randomly after packaging? And what controls are used to evaluate possible shipping and storage conditions?

We take a lot for granted - most of us now purchase our products rather than compound them ourselves in the lab, so we have to be able to trust our sources and let go of some of the responsiblity. And while this is a good forum for discussion these type of problems, we still need to be careful in how we present these problems - are they random, or actual?

Mike
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