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# Title Hits Rating
1 A Practical Guide to Setting Up an IVF Lab, Embryo Culture Systems and Running the Unit 2646 0.00
Provides a complete review of the latest knowledge on how to set-up an IVF unit, detailed description of various embryo culture systems to give the best outcome in the day-to-day practice and finally the various organizational and quality control aspects The origin of the book stems from this need of present time. The authors have tried to assemble a group of international experts to make each chapter as a guide for both inexperienced and experienced IVF professionals for setting up a new clinic and running an existing unit in an efficient and effective way. The authors have adopted a systematic approach beginning with historical perspective, taking through designing, planning and constructing basic and advanced embryological laboratory, detailed overview of various laboratory procedures that are undertaken in an IVF laboratory on a day-to-day basis, the importance of quality control and quality management and future development in the embryology field. A chapter is dedicated to batching in IVF, where the editors assemble many troubleshooting tips for doing batch IVF, which is commonly practiced in many countries.
2 Oogenesis 1666 0.00
Comprehensive, visually stunning review of oocyte medicine and biology. The authors of the chapters are distinguished authorities in their respective areas of competence.  The oocyte is the largest and most mysterious cell in the body of mammalian organisms. Through its growth and maturation, it reaches extreme levels of specialization, while maintaining at the same time a condition of totipotency. Its unique ability, in co-operation with the spermatozoon, to give rise to a fully developed organism formed from hundreds of different tissues and myriads of individual cells has inspired intellectuals of all ages. Oogenesis finds impetus and purpose in casting scientific perspective towards this unique cell for the benefit of scientists and assisted reproductive technology (ART) specialists. The authors of the chapters are distinguished authorities in their respective areas of competence. From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development.
3 Fertility Lab Insider 2062 0.00
Carole C. Wegner, PhD, HCLD shares her insider’s view of the fertility lab with patients who are considering or already in treatment for infertility. Dr. Wegner worked as an embryologist and IVF lab director over fifteen years. Her experience and knowledge of IVF lab procedures informs her advice to patients on how to find the best fertility programs and get the best healthcare from fertility clinics. Chapters in this book include, “I’m not getting pregnant, Now what?”, Getting Ready for IVF, Diagnosis and Choosing a Treatment Plan, Paying for IVF, Behind the Lab Door, Freezing Eggs, Sperm and Embryos, Cycles that Go Wrong, New Technologies Bring New Questions, Treatment Checkpoints and Patient Tools. An Embryo Photo Gallery, Glossary, Insurance Codes and a list of suggested questions for your fertility doctor are all included in the Patient Tools chapter. Additional resources are available throughout as links to websites as well as news articles and interviews, if your e-book reader supports Internet surfing.
4 Sperm Chromatin- Biological and Clinical Applications in Male Infertility and Assisted Reproduction 3717 0.00
Sperm DNA damage is common and has been associated with reduced rates of conception, impaired embryonic development and increased risk of miscarriage. Although the exact causes of sperm DNA damage are unknown, it is clear that infertile men possess substantially higher levels of sperm DNA damage than do fertile men. Written by leading, internationally renowned clinicians and basic scientists with expertise in sperm DNA, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction provides readers with a thoughtful and comprehensive review of the biological and clinical significance of sperm DNA damage. The work covers the fundamental principles of sperm chromatin architecture and function, the proposed modes of DNA damage and repair, the tests of sperm DNA damage, the clinical aspects of DNA damage and the impact of DNA damage on reproductive outcome. Unlike any other title on the topic, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction is an invaluable addition to the literature and will serve as an indispensable resource for basic scientists with an interest in sperm biology and for urologists, gynecologists, reproductive endocrinologists, and embryologists working in the field of infertility. Table of contents: I. Human Sperm Chromatin: Structure and Function 1. Sperm Chromatin: An Overview Rodney Balhorn 2. Spermatogenesis: An Overview  Rakesh Sharma and Ashok Agarwal 3. Sperm Nucleoproteins Rafael Oliva and Judit Castillo 4. The Relationship Between Chromatin Structure and DNA Damage in Mammalian Spermatozoa Kenneth Dominguez, Chris D. R. Arca, and W. Steven Ward 5. Chromosome Positioning in Spermatozoa Andrei Zalensky, Olga Mudrak, and Irina Zalenskaya 6. Sperm Mitochondrial DNA Justin C. St. John and Bianca St. John 7. The Sperm Epigenome Donovan Chan and Jacquetta Trasler 8. RNA Expression in Germ Cells During Spermatogenesis (Germ Cell Transcriptome)  Tin-Lap Lee, Albert Hoi-Hung Cheung, Owen M. Rennert, and Wai-Yee Chan II. Laboratory Evaluation of Sperm Chromatin 9. Sperm Chromatin Structure Assay (SCSA®): 30 Years’ Experience with the SCSA® Donald P. Evenson 10. Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications Jaime Gosálvez, Carmen López-Fernández, and José Luís Fernández 11. Basic and Clinical Aspects of the Sperm Chromomycin A3 Assay Giancarlo Manicardi, Davide Bizzaro, and Denny Sakkas 12. Cytochemical Tests for Sperm Chromatin Maturity Igor Tsarev and Juris Erenpreiss 13. Acridine Orange Test for Assessment of Human Sperm DNA Integrity Alex C. Varghese, C. Fischer–Hammadeh, and M. E. Hammadeh 14. Laboratory Evaluation of Sperm Chromatin: TUNEL Assay Rakesh Sharma and Ashok Agarwal 15. The Basic and Clinical Aspects of the Sperm Comet Assay Luke Simon and Sheena E. M. Lewis 16. Assays Used in the Study of Sperm Nuclear Proteins Timothy G. Jenkins, Benjamin R. Emery, and Douglas T. Carrell 17. Sperm Epigenetic Profile Cristina Joana Marques, Alberto Barros, and Mário Sousa 18. Clinical Significance of Sperm RNA Jean-Pierre Dadoune, Isabelle Galeraud-Denis, and Serge Carreau III. Biological and Clinical Determinants of Sperm Chromatin Damage 19. Role of Oxidative Stress in the Etiology of Sperm DNA Damage R. John Aitken and Geoffry N. De Iuliis 20. Abortive Apoptosis and Sperm Chromatin Damage Hasan M. El-Fakahany and Denny Sakkas 21. Spermiogenesis in Sperm Genetic Integrity Marie-Chantal Grégoire, Frédéric Leduc, and Guylain Boissonneault 22. Male Subfertility and Sperm Chromatin Damage Mona Bungum, Aleksander Giwercman, and Marcello Spanò 23. Aging and Sperm DNA Damage Fábio F. Pasqualotto and Eleonora B. Pasqualotto 24. Cancer in Males: Implications for Sperm Quality, Fertility, and Progeny Outcome Peter Chan and Bernard Robaire 25. Sperm Chromatin and Environmental Factors Aleksander Giwercman 26. Effects of Male Accessory Gland Infection on Sperm Parameters Aldo E. Calogero, Sandro La Vignera, Rosita A. Condorelli, Rosario D’Agata, and Enzo Vicari IV. Sperm Chromatin and Assisted Reproductive Technology Outcomes 27. The Impact of Sperm Processing and Cryopreservation on Sperm DNA Integrity Dan Yu, Luke Simon, and Sheena E. M. Lewis 28. Structure of Chromatin in Human Sperm Bound to Hyaluronic Acid: The Benefits of PICSI Dish Mediated Sperm Selection Leyla Sati and Gabor Huszar 29. Electrophoretic Sperm Separation Steven Fleming and R. John Aitken 30. Antioxidants and Sperm DNA Damage Armand Zini and Maria San Gabriel 31. Sperm Chromatin and ART (IUI, IVF, and ICSI) Pregnancy Mona Bungum 32. Sperm DNA Damage and Pregnancy Loss After IVF/ICSI Armand Zini and Jason Matthew Boman 33. Postnatal Effects of Sperm Chromatin Damage Miriam Pérez-Crespo, Raúl Fernández-González, Miguel Ángel Ramírez, Eva Pericuesta, Alexandra Calle, and Alfonso Gutiérrez-Adán 34. Evaluation of Chromatin and DNA Integrity in Testicular Sperm Armand Zini and Naif Al-Hathal V. Protocols and Integrity Tests 35. The Alkaline Comet Assay Sheena E. M. Lewis 36. Sperm Chromatin Structure Assay (SCSA) Donald P. Evenson 37. Measurement of DNA Damage in Spermatozoa by TUNEL Assay Rakesh Sharma and Ashok Agarwal 38. Clinical Utility of Sperm DNA Integrity Tests Armand Zini
5 Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices 6003 0.00
This unique, practical title provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists who are interested in the subject. The manual also offers an excellent description of novel procedures that will likely be employed in the near future. Table of Contents: 1. Journey of Human Gametes In Vitro: 1978 to 2010Zeev ShohamI. Setting Up and Running an IVF Laboratory2. Building the LaboratoryDean E. Morbeck and Marlena Duke3. Air Quality ManagementJohan Guns, Ronny Janssens, and Martine Vercammen4. Organizational Aspects of the Laboratory in a Tertiary Care ART CenterNancy L. Bossert and Christopher De Jonge5. Quality Control ManagementWilliam R. Boone and H. Lee Higdon III6. Daily, Weekly, and Regular Preparations for the IVF LaboratoryMichael A. Britt and Klaus E. Wiemer 7. Risk and Safety in the IVF ClinicPeter Sjoblom and Julius HreinssonII. IVF Laboratory Equipment and Culture Systems8. Essential Instruments and Disposable Supplies for an IVF Laboratory Leslie Weikert, Christa Fralick, and Klaus E. Wiemer9. Co2 and Low-O2 IncubatorsMarius Meintjes10. IVF WorkstationsNicolas Prados and Alex C. Varghese11. Culture Media in IVF: Decisions for the LaboratoryJason E. Swain and Thomas B. Pool III. In Vitro Fertilization12. Oocyte DenudingRoberta Maggiulli, Filippo Ubaldi, and Laura Rienzi13. Assessment of Oocyte Quality Basak Balaban, Turgay Barut, and Bülent Urman 14. Polarization MicroscopyMarkus Montag, Maria Köster, and Hans van der Ven15. Cumulus Cell Gene Expression in Assessment of Oocyte Quality Dagan WellsIV. Embryo Culture Methods16. Short Culture: Day 1/Day 2/Day 3 Embryo CulturePatrick Quinn17. Extended Culture in IVFDavid K. Gardner and Michelle Lane18. In Vitro Maturation of Human Oocytes Baris Ata, Jack Huang, and Ri-Cheng Chian19. In Vivo Embryo Culture DeviceClaude Ranoux20. Microfluidics for Gamete Manipulation and Embryo CultureGary D. Smith, Charles Bormann, and Shuichi TakayamaV. Sperm Processing and Selection21. Sperm Assessment: Traditional Approaches and Their Indicative Value Margot Flint, Fanuel Lampiao, Ashok Agarwal, and Stefan S du Plessis22. Sperm Assessment: Novel Approaches and Their Indicative ValueDe Y. Liu, Harold Bourne, C. Garrett, G. N. Clarke, Shlomi Barak, and H. W. G. Baker23. Sperm Processing for IVFRalf Henkel24. PESA/TESA/TESE Sperm Processing Sandro C. Esteves and Sidney Verza, Jr.25. Processing Sperm Samples in HIV-Positive PatientsThamara Viloria Samochin, Marcos Meseguer, Antonio Pellicer, José Remohí, and Nicolás Garrido26. Intracytoplasmic Morphologically Selected Sperm InjectionP. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer, Delf Schwerda, Astrid Stecher, Barbara Wirleitner, Martin Zintz, Bernard Lejeune, S. Vanderzwalmen, Nino Guy Cassuto, Mathias Zech, and Nicolas H. Zech 27. Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories Gabor Huszar28. Electrophoretic Sperm SeparationSteven Fleming and John Aitken 29. Magnetic Activated Cell Sorting of Human SpermatozoaEnver Kerem Dirican30. Poloscope-Based Sperm Selection Luca Gianaroli, M. Cristina Magli, Andor Crippa, Giorgio Cavallini, Eleonora Borghi, and Anna P. FerrarettiVI. Insemination Procedures31. Intrauterine Insemination Gautam N. Allahbadia and Rubina Merchant 32. Conventional IVF InseminationLiesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright, and Zsolt Peter Nagy33. Intracytoplasmic Sperm Injection Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi, Peter N. Schlegel, and Zev Rosenwaks34. Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed FertilizationDmitri Dozortsev and Mohammad Hossein Nasr-Esfahani VII. Micromanipulators and Micromanipulation35. Hydraulic Manipulators for ICSIHubert Joris36. Research Instruments MicromanipulatorsSteven Fleming and Catherine Pretty37. Eppendorf Micromanipulator: Setup and Operation of Electronic MicromanipulatorsEhab Abu-Marar and Safa Al-Hasani38. The Leica-IMSI SystemChristiane Wittemer, Bruno Laborde, Frederic Ribay, and Stephane Viville 39. Automated Robotic Intracytoplasmic Sperm InjectionZhe Lu, Xinyu Liu, Xuping Zhang, Clement Leung, Navid Esfandiari, Robert F. Casper, and Yu Sun40. Oocyte Treatment and Preparation for Microinjection Thomas Ebner 41. Livestock Production Via MicromanipulationAkira Onishi and Anthony C. F. PerryVIII. Embryo Evaluation, Grading, and Assisted Hatching42. Pronuclear Scoring in Human In Vitro FertilizationLynette Scott43. Cumulative Morphological Assessment of Embryo QualityBarry Behr and Aparna Hegde44. Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo ViabilityD. Sakkas, L. Botros, M. Henson, K. Judge, and P. Roos45. Oxygen Consumption as an Indicator of Oocyte and Embryo ViabilityAna S. Lopes46. Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo SelectionSamir Hamamah, Said Assou, Imène Boumela, Gisèle Ouandaogo, Hervé Dechaud, and John de Vos47. Amino Acid Turnover as a Biomarker of Embryo ViabilityRoger Sturmey, Christine Leary, Henry Leese, and Danielle Smith48. Real-time Embryo Monitoring Devices for Embryo SelectionGábor Vajta and Thorir Hardarson49. Assisted Hatching in IVFItziar Belil and Anna VeigaIX. Biopsy Procedures on Oocytes and Embryos50. Polar Body BiopsyMarkus Montag, Maria Köster, K. van der Ven, and Hans van der Ven51. Cleavage Stage Embryo BiopsyAlan R. Thornhill52. Embryo Biopsy for PGD: Current PerspectiveSteven J. McArthur, Don Leigh, Maria Traversa, James Marshall, and Robert P. S. Jansen 53. Microarrays and CGH for PGD of Chromosome Abnormalities and Gene DefectsGary Harton and Santiago MunnéX. Cryopreservation54. Sperm CryopreservationFabio Firmbach Pasqualotto, Eleonora Bedin Pasqualotto, Edson Borges, Jr., and Ashok Agarwal55. Slow Freezing of OocytesGiovanni Coticchio and Lucia De Santis56. Vitrification: Research in Animal ModelsGábor Vajta57. Oocyte VitrificationAna Cobo58. Slow Freezing of EmbryosLiesl Nel-Themaat, Ching-Chien Chang, Thomas Elliott, Patricia Bernal, Graham Wright, and Zsolt Peter Nagy59. Vitrification of EmbryosJuergen Liebermann, Joe Conaghan, Zsolt Peter Nagy, and Michael Tucker60. Directional Freezing of Reproduction Cells, Tissues, and OrgansAmir Arav61. Ovarian Tissue CryopreservationJennifer L Kulp, J. Ryan Martin, and Pasquale PatrizioXI. Embryo Transfer62. Assessment of Uterine ReceptivityHakan Cakmak and Hugh S. Taylor63. Single Embryo Transfer Thorir Hardarson and Matts Wikland64. Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based EvaluationAhmed M. Abou-Setta65. Cumulus-Aided Embryo TransferFiruza R. Parikh, Nandkishor J. Naik, Dattatray J. Naik, and Dhanajaya KulkarniXII. Management and Regulation in the ART Laboratory66. Data Management in the ART Laboratory: Requirements and SolutionsTimothy Brown and Bruce R. Gilbert67. Regulation, Licensing, and Accreditation of the ART LaboratoryDoris Baker68. Legislation in the United KingdomRachel Cutting69. Regulation, Licensing, and Accreditation of the ART Laboratory in EuropeJulius Hreinsson and Peter Sjoblom70. Regulation, Licensing, and Accreditation of ART Laboratories in India B. N. Chakravarty and Rita Modi71. Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ARTKathryn J. Go, Jay C. Patel, and Rick DietzXIII. Special Topics72. The Role of Mitochondria in the Establishment of Developmental Competence in Early Human DevelopmentJonathan Van Blerkom73. Nuclear and Cytoplasmic Transfer: Human Applications and ConcernsJosef Fulka, Jr. and Helena Fulka74. Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in FertilizationHeide Schatten, Vanesa Y. Rawe, and Qing-Yuan Sun75. Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence
6 Robert G. Edwards: A Personal Viewpoint 4301 0.00
his volume includes a unique personal history of participation in the original research in England on human in vitro fertilization. It is neither a comprehensive analysis of the career of Dr. Robert G. Edwards, the 2010 Nobel Laureate in Physiology or Medicine, nor of the writer, who is a physician-scientist expert in the field of assisted reproduction. It provides a true picture of the efforts and complexity behind an area of medical progress which has led to the births of several million babies worldwide. Not just limited to discussions of IVF, the book also illustrates some of the numerous challenges to innovation in the reproductive sciences and the difficulties of moving breakthrough reproductive technologies from the laboratory into widespread medical use.
7 Preservation of Human Oocytes 6139 0.00
A thorough analysis of current theoretical, biological, and clinical evidence generated on oocyte cryopreservation.This breakthrough title, written by experienced professionals in the field of reproductive medicine, is the must-have resource on oocyte preservation, which is quickly becoming one of the most important developments in human IVF.Certain to become the essential guide for reproductive medicine specialists, embryologists, and gynecologists, the text discusses benefits of oocyte storage for young cancer patients and women wishing to extend their reproductive lives for social, professional, or economical reasons.Topics include:• low temperature storage (cryopreservation and vitrification)• oocyte physiology• developmental stages in oocyte storage• success rates of oocyte preservation• ethics of fertility preservation• the health of babies from cryopreserved oocytes and embryos
8 Human Preimplantation Embryo Selection 17796 4.32
This book will assemble the leaders in this field to discuss their visions of dealing with the topic in the light of the limitations involving the fragility of the early human embryo. The purpose of this book is to shed light on the ever-expanding techniques that are being employed and evaluated with regard to embryo selection. A comprehensive overview may guide the ART practitioner in choice of techniques and evaluation of benefits and drawbacks. In summary, application of increasingly advanced new technology has allowed the field of embryo evaluation to evolve rapidly and dramatically over the past five years, and this book will provide an up-to-date overview and reference source.
9 Quality and Risk Management in the IVF Laboratory 10511 3.67
This essential survival guide for successfully managing the modern-day IVF clinic condenses a wealth of expertise and experience from the authors in troubleshooting and implementing quality management in the IVF laboratory. With high-profile media coverage of mistakes at IVF clinics, and escalating regulatory scrutiny, there is increasing pressure for professional accreditation. Modern accreditation schemes, which are largely based on the principles of ISO 9001 and related standards, require Quality Systems. Yet quality management beyond basic assay quality control is often poorly understood by biomedical scientists outside clinical chemistry laboratories. Quality and risk management are thus becoming hot topics for those working in IVF clinics and this book brings together, for the first time in one place, the basics of these essential aspects of laboratory management. The focus on taking a holistic approach to 'prophylactic management' - prevention rather than cure - will be welcomed by all scientists working in IVF.
10 In Vitro Fertilization: A Practical Approach 15924 4.33
Supplying a comprehensive and hands-on approach to in vitro fertilization (IVF), this source presents established state-of-the-art procedures and techniques, as well as the most current research in the field. Expert contributors explore the history of IVF and progress to future research pathways. Additional coverage includes preimplantation genetic diagnosis, oocyte retrieval, oocyte donation, and micromanipulation procedures such as ICSI, assisted hatching, and embryo biopsy.

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