ivfworld, Embryology Academy for Research
25 October 2007
Including IUI & IVF
Five days hands-on Training course on IUI, IVF, ICSI
Course Content
Day 1
Semen analysis
Semen preparation
Sperm count
Sperm morphology and motility assessment
Troubleshooting - clinical and lab related problems
Day 2
Setup of an IVF laboratory
Lab maintenance and quality management
Controlled ovarian hyper stimulation protocols
Follicular monitoring methods for optimal stimulation
Ovum pickup guidelines, identification and retrieval
Preparatory procedures for optimal oocyte handling for IVF
Media preparation and handling
Handling of pipettes
Oocyte identification and handling
Preparation of dishes for insemination
Insemination methods for IVF
Insemination of oocytes in culture dishes
Troubleshooting-clinical and lab related problems

In Vitro Fertilisation
Day 3
Different methods of sperm retrieval for ICSI
Oocyte preparation and selection for ICSI
Procedure for setting up of micromanipulator
Preparation of dishes for oocyte
Sperm immobilization and pickup
Oocyte holding and insemination
Culture of inseminated oocytes
Oocyte denudation and washing
Identification of mature oocytes
Setting up micromanipulator
Troubleshooting-clinical and lab related problems

Oocyte with Cumulus Cells
Day 4
Fertilization rate and success with different treatment protocols
Embryo culture duration, decisive factors
Embryo grading and transfer
Assisted hatching methods
Cryopreservation of gametes and embryos
Troubleshooting Assisted hatching methods
Cryopreservation of gametes and embryos
Troubleshooting Assisted hatching methods
Cryopreservation of gametes and embryos
Troubleshooting
Mature Oocyte
Day 5
ICMR guidelines
Discussion on gametes and embryo donation, sperm and ovum banking and surrogacy
Prenatal Genetic Diagnosis
Ethical aspects of assisted conception and existing laws
Current advances in ART
Further practice for micromanipulation and oocyte handling
Mock cycles for perfecting timing of procedures
Reviews, feedback, multimedia education & programme completion.
http://www.ivftraining.com/training/icsi.htm
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