| #
| Title
| Date Added
| Replies |
| 1 |
ICSI
|
13 March 2010 |
0
|
| Hey!! Please can anyone help. Sometimes when i do ICSI, after i enter the egg with the injection pipette and i aspirate some cytoplasm, when i try to release the cytoplasm back, the sperm doesnt get down and stays sticked into the injection pipette. what shall i do to avoid pvp from entering while letting the sperm down in such cases?? |
| 2 |
iui for selection of y sperms
|
12 March 2010 |
0
|
| dear all,is there any technique of spermwash for iui that can increase chances of male/female baby for the purpose of family balancing.thanks in advance dr fahmeena |
| 3 |
experienced working partner for ivf centre
|
12 March 2010 |
0
|
| dear all looking for a working partner for an ivf centre,will be commencing from june onwards at hyderabad,india,must have good experience in ivf(clinician) |
| 4 |
Minc vs ORGIO/Planer Benchtop Incubator BT37
|
02 March 2010 |
0
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| I want to buy a mini-incubator for blastocyst culture but I don’t know what to chose since I have two possibilities : one is MINC benchtop incubator from COOC, and the second option is ORGIO/Planer Benchtop Incubator BT37 from MidAtlantic. I have strong recomandation from people how work with Cook and nothing about Orgio but Orgio has a good price. Can somebody give me more details about Orgio in comparation with Cook. Thanks Mihaela R |
| 5 |
Embryo biopsy
|
18 February 2010 |
1
|
| Please can anyone suggest training for an embryologist, for cleavage stage and tropoductum biopsy .... followed by fixatation. Also, what do you suggest for blastocyst biopsy media?? Is it the same as what you are using for Day 3 biopsy?? I tried to do a day 5 biopsy but as soon as i break tropo with needle the Blastocyst collapses !! is this is normal? Finally what should I do with a hatched blastocyst? If it is hatched can I still can do a biopsy? Thank you |
| 6 |
Blasto Vitrification?
|
25 January 2010 |
1
|
| hi every one my question is which Day of culture,Blastocycst should be vitrified? Day 5 or Day 6(Extended /hatched) thank you....... |
| 7 |
oocyte denudation
|
22 January 2010 |
2
|
| hey!! I am currently having problems in oocyte denudation. I usually rely on chemical (hyaluronidase) and mechanical (moving from large stripper tip diameter to smaller one); but sometimes after moving the oocytes from hyaluronidase in order to complete the mechanical mechanism, they stick to each other and the cumulus become very hard. So they can't be aspirated with a stripper and i rely on syringes tips to release the eggs. How can i use syringes without touching the eggs? is there any specific technique? because sometimes the eggs become dead. |
| 8 |
retained embryos
|
20 January 2010 |
2
|
| what could be the possible reason for retained embryos after the transfer, we came across this event in few successive procedures which is othervise an extremely rare event in our IVF/ICSI-ET programme. |
| 9 |
Stability of pH during media equilibration
|
12 January 2010 |
1
|
| Dear All, I would like to know if anybody has monitored the stability of medium pH during equilibration ( in our laboratory we use G1 v5 PLUS, Vitrolife and pH Meter Version 2 from RI). We have done 4 24-hours measurements: 1) in two cases after 8 hours of equilibration pH-meter showed the minimal value and this reading was stable for next 8 hours. Then pH increased at about 0,03-0,04 in the last 8-hours of measurement. 2) in the next two cases after 8 hours of equilibration pH-meter showed the minimal value but this reading was stable only for 2-3 hours and then the pH increased at about 0,03-0,04 too. Has anybody noticed a similar tendency ? |
| 10 |
artifacts in culture dishes.
|
24 December 2009 |
2
|
| Hi guys. sometimes we see artifacts in our culture dishes. we use BD falcon 7575 to aliquote medium and prepare culture dishes. when you open individually packed 7575 pippettes, lot of particles are produced, which you can see in the image. we suspect we see the same particles in our culture dishes. Anyone having this problem? Pls comment and share your experience on this. |
| 11 |
DNA Fragmentation Test For Sperm
|
19 December 2009 |
3
|
| Dear All. I want to know about your experience with DNA fragmentation kits. Secondly I am interested in experiences with intravaginal culture (Invo-cell) technique. Thank you in advance seeking internation members comments/observation. |
| 12 |
Time of drug application
|
15 December 2009 |
1
|
| According to knowledge from traditional Chinese medicine, proper time of drug application is very important for better outcome.What I mean is for example Menopur or any other type of drug used for induction of ovulation is it better to be taken at 1 p.m. instead of 2.a.m. Do you have any experience in this. In advance , Thanks Bojana |
| 13 |
Air injection system v/s Oil injection system
|
08 December 2009 |
0
|
| Is there any difference in fert. rate with air injection system compared to oil injection system. The difference i have seen is that the aspiration of oolema is much smoother, much better control with oil injection system. The rupture of oolema with air system is very traumatic at times. Really worries. Anybody using Air sytem, have any tips suggestions to share. pls comment on how to ensure convenient and atraumatic injection with air injection system |
| 14 |
Testicular biospy red blood cells
|
05 December 2009 |
1
|
| Could you please share your experience with bloody testicular samples? Have you try Spermprep colums of red blood lysis? Thanks Ivan |
| 15 |
oocyte in vitro maturation
|
03 December 2009 |
2
|
| I have question according to in vitro maturation of imature oocyte after ordinar stimulation protocol and OPU. If anybody have experience of maturation of oocyte in vitro when only after denudation you observe that lot of oocyte are MI? |
| 16 |
Using single media to day 5 . fertsert paper
|
20 November 2009 |
1
|
| Just read an interesting paper using single continuous media to blastocyst. I noticed that embryos were cultured in a low O2 environment. Does anyone know if low O2 tension helps to minimize release of possible toxic compounds into the media or would the results likely be the same at ambient O2. Any thoughts? |
| 17 |
Retired gynaecologist expert in IVF, clinical and laboratory work available for cooperation with IVF center
|
18 November 2009 |
0
|
| Is there anybody in need of a specialist in IVF, clinical and laboratory work? I am an italian gynaecologist performing IVF and related procedures since 1980. |
| 18 |
DNA fragmentation for sperm
|
16 November 2009 |
1
|
| Dear All, could you please advice me which company is produced PCR kits (Conventional or Real-Time)to be used for SPERM DNA FRAGMENTATION Thank in advance Ayman |
| 19 |
Clevage failures
|
13 November 2009 |
2
|
| I am working in bovine OPU IVP. and I have some problems with fertilization when checking cleavage rate at D2 after IVF where no more than 40% is achieved. I had checked fertilization medium ph at begining and ending (7.6 and 7.5) of 18 hs of IVF (TALP Fertilization medium), semen has been already used before and works well, incubator is pretty new but I dont really know how acurrate is CO2 injection and concentration, bicarbonate concentration in media could be not enough to equilibrate? any suggestions? Thanks in advance |
| 20 |
No Cleavage after Fertilisation.
|
13 November 2009 |
2
|
| Hi, I had this problem recently a couple of times, fert check at 18 hrs post ICSI showed 14 ova had 2pn but at 48 hrs only 7 had reached 4 cell stage, no cleavage in the rest, no pn either. I cultured the embryos further to 72 hrs, then 2 more embryos cleaved to 2 cell stage, no development in the rest. Other patients embryos which were cultured during the same time and in similar conditions progressed very well at 48 hrs. Checked everything from temp to Carbon dioxide saturation everything found ok. Second time around same thing occurred, only 50% of fertilised embryos cleaved in one patient whereas other patients embryos developed well. All the patients were given the same drugs and no age factor (both patient below 30 yrs). what may be the reason behind this? |
