IVF Mail - Loading the embryo transfer catheter

Loading the embryo transfer catheter

By: mai galal, horysivf center
01 January 2005


Question:
	What is the ratio of air to media in the catheter and its amount?

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Visitors replies (4)

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damian pike said on 09 August 2007

"Hi,

different folks use different methods :)

We use the sidney ivf ( similar to the labotec) and are very happy with it : stiff outer & small diameter inner.

I like to load the catheter with media -this "wets" the syringe etc & also media being water based is non-compressible so you get no pressure changes when the catheter is loaded / unloaded.

I load the embryos in as small amount of media as possible. I then draw up a small bubble of air -then "cap" the catheter with an small bubble of media.

I have been getting good rates for a number of years. but i`m always open to new ideas :)

Good luck

Damian"

Derek said on 23 February 2005

"It also depends on what catheter you are using. Labotect has a recomended protocol of using their catheter. Just put enough media to deliver the embryo. Because of you put too much then you will only flush it out of the uterus. There is no need to put a lot of air. This works for me."

Pasha said on 01 January 2005

"It is not necessarily at all to fill the ET catheter with an air?
We mount the embryos without air in 50 ul of medium, that contain 20% of synthetic serum supplement (in order to prevent sticking of an embryos to the catheter walls)."

Fernando Prados said on 01 January 2005

"Dr. Galal, we fill the catheter with medium. Before starting to pick up the embryos, we take a small air bubble (twice or three times the catheter width). Then, we take about 6 microl. of medium and start picking embryos (we try to take them separately). After the last embryo is taken, we aspirate 6 more microl. and another small air bubble. The final total volume is around 15 ? 20 microl.
Regards,
Fernando Prados"