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Loading the embryo transfer catheter

By: mai galal, horysivf center
01 January 2005
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Question:
What is the ratio of air to media in the catheter and its amount?
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 Replies (4)
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Hi,

different folks use different methods :)

We use the sidney ivf ( similar to the labotec) and are very happy with it : stiff outer & small diameter inner.

I like to load the catheter with media -this "wets" the syringe etc & also media being water based is non-compressible so you get no pressure changes when the catheter is loaded / unloaded.

I load the embryos in as small amount of media as possible. I then draw up a small bubble of air -then "cap" the catheter with an small bubble of media.

I have been getting good rates for a number of years. but i`m always open to new ideas :)

Good luck

Damian
damian pike - 09 August 2007


It also depends on what catheter you are using. Labotect has a recomended protocol of using their catheter. Just put enough media to deliver the embryo. Because of you put too much then you will only flush it out of the uterus. There is no need to put a lot of air. This works for me.
Derek - 23 February 2005


It is not necessarily at all to fill the ET catheter with an air?
We mount the embryos without air in 50 ul of medium, that contain 20% of synthetic serum supplement (in order to prevent sticking of an embryos to the catheter walls).
Pasha - 01 January 2005


Dr. Galal, we fill the catheter with medium. Before starting to pick up the embryos, we take a small air bubble (twice or three times the catheter width). Then, we take about 6 microl. of medium and start picking embryos (we try to take them separately). After the last embryo is taken, we aspirate 6 more microl. and another small air bubble. The final total volume is around 15 ? 20 microl.
Regards,
Fernando Prados
Fernando Prados - 01 January 2005

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