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mink incubator

By: george, mission hospital
05 January 2008

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Question:
	Hi, At our center we are know going to use cook mink incubator.Can somebody help me to solve the problem,that we are at 553 m above the sea level so how much co2 should be used at this level at present we are using 5.0 %co2 so please help me to solve the problem Thanking you

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Orestes said on 16 January 2008

"I recomend you not to matter about co2 concentration.Just strive reach em PH around 7.3 irrespective of medium that you use.
Read a very good review, by Thomas Pool, at http://www.irvinesci.com/techinfo/mediareview1.pdf

best regards

Orestes prudencio MD"

Dr Paul Knaggs said on 07 January 2008

"Hi George

With a minc you will have to run with a premixed gas so I don't think atmospheric effects will be much of a problem - the CO2 concentration will depend on you media and what the manufacturers recommend to give their optimal pH. If you know what pH you want to use calculate the required CO2 concentration using Henderson- Hasselbach. With the Cook medium I have experience of, I used 6%CO2 5%O2 and 89%N2. feel free to email me if you require any further help
Regards

Paul"