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mink incubator |
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george, mission hospital
05 January 2008
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Hi,
At our center we are know going to use cook mink incubator.Can somebody help me to solve the problem,that we are at 553 m above the sea level so how much co2 should be used at this level at present we are using 5.0 %co2 so please help me to solve the problem
Thanking you
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I recomend you not to matter about co2 concentration.Just strive reach em PH around 7.3 irrespective of medium that you use.
Read a very good review, by Thomas Pool, at http://www.irvinesci.com/techinfo/mediareview1.pdf
best regards
Orestes prudencio MD
Orestes - 16 January 2008
Hi George
With a minc you will have to run with a premixed gas so I don't think atmospheric effects will be much of a problem - the CO2 concentration will depend on you media and what the manufacturers recommend to give their optimal pH. If you know what pH you want to use calculate the required CO2 concentration using Henderson- Hasselbach. With the Cook medium I have experience of, I used 6%CO2 5%O2 and 89%N2. feel free to email me if you require any further help
Regards
Paul
Dr Paul Knaggs - 07 January 2008
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