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Emb. Lab. Dırector
Joy Ege said on 10 November 2009
"Different culture systems have to be gassesed experimenatlly. For Vitrolife 4 hours is enough without oil overlay.
We usually gas culture dishes minumun with oil 8 hours.
Yours truly.
Emb. Dr. Joy Ege"
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Off gassing dishes for IVF
Michael L. Reed said on 08 November 2009
"A good basic question - years back there were many papers on ethylene oxide residue and the need for off-gassing, but those days are essentially behind us.
Now we have irradiated plastics, sealed in plastic. And most of us would agree that you can smell the " -ene " gasses when you open a new pack of dishes. I have a few older references that might give recommendations, but I don't recall seeing that information - just general comments about airing the dishes out.
Do dishes ever stop off-gassing? We store them at room temperature, then heat them up. But in the incubator, there are scavengers that do help - water pans, oil overlay if used, filtration systems if used.
I don't have a good answer, sorry.
Here are a few older references -
Gilligan, T, Schimmel, T, Esposito Jr., B, Cohen, J 1997 Release of volatile organic compounds such as styrene by sterile petri dishes and flasks used for in-vitro fertilization.
Fert Steril 68;S52-S53
Hall, J, Gilligan, A, Schimmel, T, Cecchi, M, Cohen, J 1998 The origin, effects and control of air pollution in laboratories used for human embryo culture. Hum Reprod 13;146-155.
Cohen, J, Gilligan A, Esposito W, Schimmel, Dale, B 1997 Ambient air and its potential effects on conception in vitro. Hum Reprod 12;1742-1749.
Mike"
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