 |
|
 |
 |
 |
 |
CategoriesSponsorsProduct ReviewsIVF Journals
Recently Viewed
|
|
Latest Worldwide IVF Jobs
 Leeds MSc Course
Free NewsletterFeatured
Most PopularLatest |
||||||||||||||||||||||||||
| Search Listings | Place Listings | Edit Listings | My Profile | My Favorites |
Auto Notify |
Sitemap | FAQ | Terms of Use | Privacy Policy | Contact Us | Tell Your Friends | Refund Policy | ROR/RSS | Sponsorship and Advertising Copyright © 1997-2012, IVF.net. All rights reserved worldwide. |
Oocyte denudation
Zankruti said on 28 January 2010
"Hey,
You can always try washing the COCs in gamete buffer before putting them into Hyal.
It actually dilutes n washes away lot of things like excess cumulus cells, follicular fluid etc.
Also you can tease the COC while pick up with the pasteur pipettes leaving behind the oocytes covered with 7-9 layers of cells. It really help in stripping and no effect on fertilization and further development."
Reply
Afsaneh said on 26 January 2010
"Important points to avoid problems at the time of denudation:
1. collect the egg solely, avoid to transfer follicular fluid with it.
2. collect the eggs in a center well dish in the begining, then try to transfer them to another drop (for example a drop outside the middle circle) and again to another drop, so the eggs will be really free of follicular fluid. You can use the other methods of washing.
2. If it is possible for you, try to cut the extra cumulus with needle.
3. transfer the eggs to a drop of hyalu. and leave them there only for 5 sec. then transfer them to another hyalu. drop and do cleaning by pipetting."
Reply