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oocyte in vitro maturation

By: Monta,
03 December 2009
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Question:

I have question according to in vitro maturation of imature oocyte after ordinar stimulation protocol and OPU. If anybody have experience of maturation of oocyte in vitro when only after denudation you observe that lot of oocyte are MI?

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Dr.Saritha K.R


MI oocytes to healthy baby.
Dr.Saritha K.R said on 10 April 2010

"We had many such cases. Within 4- 6hours most of the MI will change in to MII in fertilization media itself. ICSI done on such MII oocytes showed fertilization, cleavage, pregnancy following embryo transfer and delivered normal healthy baby. Normally we do Day 3 transfer. Ootyes which mature to MII on next day of retrieval though turns to beautiful embryos following ICSI, pregnancy never occurred after embryo transfer.
Dr.Saritha"

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Ritsa Storeng


Director of IVF laboratory
Ritsa Storeng said on 08 December 2009

"2: oocyte in vitro maturation

Yes, we observe MI oocytes upon denudation and after 2-3 hours in vitro we observe mature MII oocytes. We do perform ICSI and on several occasions embryo transfer was performed with nice 4-cell embryos obtained after ICSI with in vitro matured MI to MII oocytes. I have not data on pregnancies or live births after such transfers."

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Hady Zidan


immture oocyte after OPU
Hady Zidan said on 07 December 2009

"You can use IVM media for culture for a day and observe the next day"

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