IVF Mail - Oocytes elasticity

Oocytes elasticity

By: Serj,
09 February 2008


Question:
	Greetings! Somebody knows, why at performance ICSI sometimes cells elastic and resistant and all is wonderful, but sometimes very soft and it is necessary to pierce cells with the big efforts of them, very cautiously to not pierce an opposite wall. On what it can depend?

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Dr. Dharmesh Kapadia said on 15 February 2008

"Dear Friend,
Apart from given causes , if you do icsi after longer incubation of occs in culture media then the zona gets thickened and the oolemma becomes elastic and it can happen to pierce the opposite wall while injecting"

Linda Hoover said on 11 February 2008

"Regarding poor integrity of the oolemma: We have been finding that this is often related to PCOS-type patients with high FSH, possibly those on Metformin. We are in the process of investigating this futher. Any information you discover on your end would be appreciated as well. Thanks."

Dr.Ravindranath said on 11 February 2008

"Almost every Embryologist experience in ICSI what you have experienced. Due to controlled hyperstimulation, many oocytes gets to maturity though naturally they would have withered. In a cohort, the oocytes do show asynchrony and hence different types of oocytes with different types of ooplasms etc. Thats why, the priming of the patients is important before taking up for ICSI. For ex. the PCOS patients need be investigated and treated for the pathology they carry or else we will end of with bad quality oocytes and can face what you are facing during ICSI. So, don't get annoyed by bad eggs, it's natural but do tell your clinician to prime the patient with appropriate gonadotrophins. Thanks."

Mohammed Ibrahim said on 09 February 2008

"I had a bad experience with such oocytes. you have to be very gentle during ICSI. it might be related to stimulation period, the pressure at which oocytes were aspirated.
all the best."