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Optimum CO2 level
Patrick Quinn said on 18 October 2009
"Michael Reed has touched on the major points in his response to this post. CO2 is important but the more important thing to measure is the pH of the medium, which, of course is controlled by CO2 levels and also the concentration of NaHCO3 in the medium, altitude, temperature, being the primary factors. Keep in mind to that each incubator in a lab can vary with regard to measured pH at the same CO2 level. Do you measure pH? If not, then you are doing your patients a diservice.
See the paper by Quinn & Cooke 2004, Fertil Steril Vol 81 pp 1502-6 for further details,. and also the first two refernces in that paper, namely:
1. Quinn P. Media used in the assisted reproductive technologies laboratories.
In: Patrizio P, Tucker MJ, Guelman V, eds. A color atlas for
human assisted reproduction. Laboratory and clinical insights. Philadelphia,
PA: Lippincott Williams & Wilkins, 2003:241–56.
2. Mortimer D, Quinn P. Bicarbonate-buffered media and CO2. Alpha
Newslett 1996;April."
Reply
Optimum CO2
Michael L. Reed said on 14 October 2009
"Back to the basics - how are you measuring your CO2, and what is the pH of the media, with and without protein supplementation at 6.7% CO2? Are you targeting a specific pH range, or just using the embyrology to determine CO2%?
And if you are happy with your embryology and clinical pregnancies under these conditions, you could target the same pH with Cook media to start with, regardless of what the company states. I would definitely do pH trials before placing any embryos into the Cook media.
Also ask Cook how they determined the recommended pH - were they just targeting a certain pH, and do they have clinical data to back it up - and what altitude and laboratory conditions did they encounter while determining their pH values?
Within patient comparisons, splitting the embryos up for each patient into the two separate media, could be useful as well - as long as the pH of both media are similar. Donor egg cycles are a good place to start for this.
Good luck!
Mike"
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