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Out of incubator time

By: Jim,
18 August 2008
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Question:
Can some kind person advise on detail re current thoughts on how long dishes (w and w/o oil) should be out of the incubator to minimise ph and temp changes, and any controls in the lab they have been able to setup. Ta

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It depends on the type of buffer your media using if with hepes it will last long as 2 or more hours if it is bicarbonate buffer,with oil it has to be back to the inc. within 2 minutes without oil as less as 5-10 seconds then back to the inc.
tom - 21 August 2008

 






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