IVF Mail - Poor embryo survival after thaw

Poor embryo survival after thaw

By: May,
09 June 2006


Question:
	Hello, just a quick question. Recently, our embryo survival rate has not been reasonable. MOst embryos thawed were lysed. All embryos were of good day2 quality. What could be the reason as none of the protocol has been changed except substituting IMV Tech embryo straws with Cryo Bio System embryo freezing straws. Anybody has this kind of problem?

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May said on 16 November 2006

"Hie Mike. Thank you for your comments. I understand your freezing technique as I'm doing the same thing as you are. I also load media first, then air then media with embryos, air and seal it off with some media only. Seeding is done at the upper part of the first column of media near the cotton plug. I also use a cotton swab for seeding. Recently, I did some thinking and researching and also found out that CBS straws are as safe as IMV tech straws. Thank you so much for your advice."

subir chatterjee said on 21 June 2006

"i think during seeding some problem are occaring. i am using cryo bio system straw and plug. load media first - air gap - media with embryo - air gap - media, press sealing plug.run your system, seed with cotton swab and just bottom of the plug, that means far distant from your embryo.follow all other steps carefully. i hope you will get result."

Michael L. Reed said on 10 June 2006

"May: I'd suggest first looking at the numerous comments on EmbryoMail, regarding the CBS straws. You could be facing a learning curve with the change to the new straws. You need to be very aware of where the embryos are within the straw when you seed - if you use the loading nozzle/tip, the embryos may be scattered if you draw them into the straw too fast. I actually load the embryos using a flexible pipet tip, rather than the nozzle/tip provided. I can see exactly where I'm placing the embyros that way. Also, you should check to see that the straws seed correctly, and remain seeded (larger cross sectional volume and different straw material). I've found that a cotton tip swab soaked in LN2 seeds the CBS straw better than the metal rod I was using successfully for the IMV straws. I've had very good clinical success with the CBS straws, as have others, so it may be that some minor changes in technique are needed, rather than changes in the freezing protocol. Good luck. Mike"