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Seeding position in straw

By: wendy,
31 July 2008
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Question:
I would like to ask where is the position of straw for seeding during embryo freezing? seeding in cotton head with freezing medium or seeding in embryo column directly?
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Wendy



Wendy said on 09 August 2008

"Thank you"

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Simon Aiken



Simon Aiken said on 04 August 2008

"Depending on how you're loading the cryostraws, you normally seed away from the embryo.

If say (going from top to bottom) you have freeze media/air gap/freeze media with embryo/air gap/freeze media; you'd seed the top freeze media section."

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