Dear All,
I would like to know if anybody has monitored the stability of medium pH during equilibration ( in our laboratory we use G1 v5 PLUS, Vitrolife and pH Meter Version 2 from RI). We have done 4 24-hours measurements:
1) in two cases after 8 hours of equilibration pH-meter showed the minimal value and this reading was stable for next 8 hours. Then pH increased at about 0,03-0,04 in the last 8-hours of measurement.
2) in the next two cases after 8 hours of equilibration pH-meter showed the minimal value but this reading was stable only for 2-3 hours and then the pH increased at about 0,03-0,04 too.
Has anybody noticed a similar tendency ?
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That is a fine observation
ALOK Teotia said on 15 January 2010
"The observation is appreciable, which PH meter are you using, and what was the volume and container of the media. did u measured the ph while inside the co2 incubator and what was the set co2 concentration (%), was that a triple gas or a single gas incubation. please detail the procedure of measurement.
i hope ph control for IVF cultures is worth a long discussion. I will request the experts for the same."
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