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Stem Cell Workshop

Dr. Orly Lacham-Kaplan, Senior Research Fellow, MISCL, Stem Cell Laboratories (MISCL) of Monash University, Melbourne Australia
02 July 2007
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Dear Colleagues and students,
Don’t miss the opportunity to attend to the Summer course in stem cell research at The Monash Immunology and Stem Cell Laboratories (MISCL) of Monash University, Melbourne Australia
Professor Alan Trounson, Director                                                     
 
Dates: January 7th – February 3rd, 2008
Attached you will find detailed information.
The application form and preliminary program are available upon request.         


  

Stem Cell Workshop


Summer course in stem cell research at The Monash Immunology and Stem Cell Laboratories (MISCL) of Monash University, Melbourne Australia
Professor Alan Trounson, Director                                                      

Dates: January 7th - February 3rd, 2008

A four week course at the Monash Immunology and Stem Cell Laboratories (MISCL) of Monash University, Melbourne, Australia, will be held in January 2008 and will include lectures and hands-on laboratory work. The course is suited for biology and biotechnology scientists who are seeking advance exploration of techniques associated with research in stem cells. Accommodation, social events and outings are included.

The course includes lectures and seminars by experts in the fields of embryology, stem cell biology, and biotechnology. The lectures will focus on human embryology, nuclear reprogramming, maintenance and differentiation of ES cells, and application to regenerative medicine, and ethics and business in the world of stem cells.

Practical sessions are held at MISCL. Participants will be involved in a weekly rotation in specified laboratories. Practical sessions will be introduced and guided by resident scientists, postgraduate students and staff members. Participants will be given a one on one tutoring. Practical work will include instructions of tissue culture techniques for embryos and stem cells, imaging and molecular analyses.

The course will be divided into 4 major sections:
1. Nuclear transfer and cell fusion. This part will include production of mouse embryos following enucleation and injection or fusion of somatic and ES cells.
2. Maintenance of mouse and human ES cells. This part will include culture of undifferentiated ES cells, and splitting and freezing ES cells.
3. Identification of ES cells. This part will include:  identification of specific markers for human and mouse embryonic stem cells by microscopic (immuno fluorescent antibodies) and molecular (RT-PCR) methods. 
4. ES cells differentiation:  This part will include formation of embryoid bodies and the identification of selectable markers for differentiated cells.

Laboratory work will be supplemented by seminars on the protocols used during the practical work. 

To apply contact course coordinator:
Dr. Orly Lacham-Kaplan, Senior Research Fellow, MISCL
orly.lacham-kaplan@med.monash.edu.au


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