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sterilisation of embryo biopsy needles

By: carolyn Cummins,
22 February 2008
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Question:

I have been told that ideally embryo biopsy needles need to be washed in deionised water followed by an acid-wash before sonication. Does anyone know what should be used for the acid-wash? Dilute bleach or related substance or does a particular acid have to be used? Thank you

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Sevinc Ege



Sevinc Ege said on 10 March 2008

"Glass pipettes should be prewashed with 3%HCL for 24 hrs. They should then be rinsed in RO water .."

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