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Testicular biospy red blood cells

By: Ivan Valencia, endogyn
05 December 2009
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Question:

Could you please share your experience with bloody testicular samples? Have you try Spermprep colums of red blood lysis?

Thanks

Ivan
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Roxana Napolitano


Testicular biopsy red blood cells
Roxana Napolitano said on 11 March 2010

"I don't use any lysis buffer.I strongly grab the tiny piece of tissue with a gauze and I squeeze it.Then I wash the piece of tissue with PBS. I repeat these 2 steps 3 times.
Finally I rend the tissue using two slides."

Reply

Nidaa.J


erethrocyte lysis buffer
Nidaa.J said on 25 December 2009

"I use Lysis buffer which i prepare myself, ammonium chloride 4.495 gm + pottassium bicarbonate 0.5 gm + EDTA 0.0185 gm all dissolved in 50 ml distilled water and filtered, during processing of the sample with sperm medium i add anappropriate volume of the buffer, and it does pretty well

Good luck"

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