Does anybody use bactericides and fungicides in waterbath of incubator? These have any negative effects on embryo and sperm? Unless harmfull then which trademark can be recommended?
The use of copper metal in a water bath/water pan is a great idea. It is cheap and not volatile so it can't transport into the embryo's media
The key to keep mold and bacteria down is basic aseptic technique. Frequent changes of the water pan with sterile reagent water also helps.
For incubator cleaning we use a dilute aqueous solution of "quats" rinsing with 70% Et-OH followed by 6% H2O2. The H2O2 breaks down to water and oxygen... Antonia Gilligan - 11 September 2008
Your can try AQUATEC which is very convenient and easy solution to contain the unwanted microbial growth in water in Instruments. May visit www.thermo.com/aquatec for details Krishnendu Roy - 29 August 2008
As fungicides I use a small copper coin or wire.
I hope this help. Orestes Prudencio - 28 August 2008
