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IVF > IVF Mail
Zeiss with plast DIC |
By:
Mona, Genesys
09 July 2010
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Dear all,
From your experience it is better to buy a Zeiss system with plast DIC and a module for IMSI to be used for bought ICSI and IMSI or to buy separate units with Hoffman for ICSI. I don't have experience with plastic DIC and in my opinion the image is hard to focus for ICSI, images on Hoffman are clearer.
What would you suggest me?
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Angela Higgs said on 15 July 2010
"I am suggesting PlasDIC for ICSI, better Zona pellucida visible, so easier to inject the sperm, more working distance of the Zeiss condenser versus the Hoffman Kondenser allows to position the needles better and easier and when doing IMSI, with PlasDIC 63x and an additional magnification of 1,6x or 2,5x, you will get to the needed magnification for seeing the vacuoles etc. in sperm. PlasDIC is a nice contrast, comparable to Nomarski (DIC), but possible with Plastic dishes, which DIc is not. HMC or Hoffman Kontrast is better to visualize the nuclei of the developing embryo, for HMC seems more like Phasecontrast, hence looks inside more. the advantage of PlasDIC for ICSI and IMSI: You can perform both in one plastic dish! Any more questions, e mail me, ok?
I am the product manager who focusses on IVF applications and have mich experience with this application, hence can help.
a.higgs@zeiss.de"
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