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# Title Date Added Replies
11 Second hand Micromanipulator needed for a centre 13 April 2012 0
Second hand micromanipulator required for an ivf clinic.. Please contact. R. Krishnamoorthy, Embryologist 09806175161 09543243777  
12 non stop continuous running of vertcal laminar flow,co2 incubator & triple gas incubator 09 April 2012 1
hai,is it a must to run the vertical laminar flow,co2 incubator & triple gas incubator,air conditioner at 26 degree centigrade & positive pressure module in ivf lab all 365 days,non stop & continuously,even if v do batches once in two months?suppose,if v run the incubator all 365 days at 37 degree centigrade with 5% c02,with 2.5 litres of water when v have to change the water,once in how many days?is it a must to run the incubator with full capacity 2.5 litrs of water or can v reduce it?
13 Need guidance 02 April 2012 0
 I hold a doctorate in human biology. I want to get trained as an embryologist. I did come across some embryology training courses which offer a week or two of hands-on training in embryological techniques. I am also aware that few UK universities offer master courses in embryology. Considering the fact that I have extensive experience in handling embryonic stem cell culture and also have a basic knowledge of embryology, which course would be apt for me if I want to become a full-time embryologist? What are the best embryology books available for embryologists? Should I do a master in embryology even if I hold a doctorate in human biology? I would be very happy if someone could offer their guidance. Thank You  
14 contamination in Ivf 28 March 2012 3
Why am I getting a lot of IVF plates with contamination? can you tell me the sources? Is density gradient a must for sperm prep for IVF?How much medium should be taken for denudation following IVF?
15 the best for AH 23 March 2012 0
hi all, i want to know about the best methode of Assisted Hatching that you choose and how about the result? i'm waiting your reply and thank you.
16 treatment of degraded sperms 22 March 2012 0
hi, i want to ask what is the recommended treatment for degraded sperms as i have performed dna fragmentation test on a patient and this is the report which i have already mentioned.
17 oocyte transport 20 March 2012 1
Dear All, Do you have any experience with oocyte trasport after retrieval ? I want to know what temprature should I maintain? Transport should be in follicular fluid ?? I wait for the answer
18 pH adjust 19 March 2012 0
I set up the pH of the medium on 7.32 , the CO2 6,5 % and the results were amaizing, there were perfect fertilization, day 3 and blast embryos. The patients gets pregnant ,but after 12 weeks they became on miscarriages (8/10 patients). I a would like to know if  the pH can be the cause and how can I solve it. Thank
19 Global Total Medium 03 March 2012 4
Dear all, We are recently having problem with Quinn's Blastocyst Medium. The embryos do well in Quinn's Cleavage medium till day 3. But they are not growing well at all in blastocyst medium. Anyone having this problem? We are planning to switch to Life Global's "Global total" It is meant to be used for culturing embryos from day 1 to Blastocyst Stage. What is your experience with this medium.
20 Airocide Air Purifier-Reg 01 March 2012 0
Expect Suggestions for using "AIROCIDE AIR PURIFIER" in IVF labs?

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