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IVF Image Database - Sperm


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1. Globozoospermia

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Globozoospermia Details:
Electron micrograph of the round-headed human spermatozoa.

Submitted by:
Andrei Rybouchkin
Views: 5799

2. Native Assessment of Sperm Ultramorphology (NASUM)

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Native Assessment of Sperm Ultramorphology (NASUM) Details:
Native Assessment of Sperm Ultramorphology - the microscope system developed in Altravita IVF Clinic employed both Hoffman and Nomarski contrast techniques. Resolution increase was attained with

Submitted by:
Alexey
Views: 6518

3. Spermatids

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Spermatids Details:
The multinuclear cell is a result of incomplete spermatogenesis resulting in undivided spermatids as an abnormal round cell. Spermatids with cytoplasm are at the stage just before 

Submitted by:
Professor Philip J. Chan
Views: 3726

4. Difference between WBC and Spermatids

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Difference between WBC and Spermatids Details:
Can you tell which image shows polymorphonuclear leukocytes(PMN) and which image shows spermatids? Answer: The left column are PMN leukocytes or white blood cells (WBC). Diff-Quik stain of semen. Ma

Submitted by:
Professor Philip J. Chan
Views: 4309

5. Simple Diff-Quik stain for chromatin integrity including DNA fragmentation.

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Simple Diff-Quik stain for chromatin integrity including DNA fragmentation. Details:
Dual use of Diff-Quik (Hema 3) for both strict morphology and chromatin integrity (Hum Reprod. 2009;24:28) can be improved (J Reprod Med. 2015;60:6) by an immediate post-stain 1-minute soak-in-water s

Submitted by:
Professor Philip J. Chan
Views: 7426

6. DNA Damage in Sperm Toroids revealed by Triton X-100 and DTT Pre-treatment before Staining.

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DNA Damage in Sperm Toroids revealed by Triton X-100 and DTT Pre-treatment before Staining. Details:
Sperm DNA compressed into toroids are exposed by acidified Triton X-100 and DTT pre-treatment (Biomed Res Int. 2015;2015:780983). DNA damage (darker color) is revealed following simple staining (Diff-

Submitted by:
Professor Philip J. Chan
Views: 7329

7. Comet Assay: DNA-Damaged Sperm

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Comet Assay: DNA-Damaged Sperm Details:
Real-time modified alkaline SCGE assay showing fluorescent DNA unraveling from damaged sperm nucleus and forming comet tail. Am J Obstet Gynecol. 2001;184:1068.

Submitted by:
Prof. Philip J. Chan
Views: 6106

8. Sperm Comet Assay (SCGE)

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Sperm Comet Assay (SCGE) Details:
DNA damage (single and double strand breaks) in alkaline comet assay is revealed by streaking tail from a diminished sperm nucleus. Fertil Steril 75:1-4,2001

Submitted by:
Dr.Philip Chan
Views: 7248

9. Sperm Head Decondensation

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Sperm Head Decondensation Details:
Composite photos of in vitro sperm head decondensation. After sperm entry into the oocyte, the head enlarges (the acrosomal cap is sometimes visible), followed by formation of the pronucleus. Magnific

Submitted by:
Prof. Philip J. Chan
Views: 9593

10. Zeta potential of sperm

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Zeta potential of sperm Details:
The mature sperm cell has a negative "zeta" electropotential after acquiring epididymal proteins and sticks to the surface at the: (1) acrosome tip (2) postacrosome (3) midpiece (4) tailtip or (5) ent

Submitted by:
Professor Philip J. Chan
Views: 7296

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